脂肪干细胞复合壳聚糖支架移植对兔退变早期椎间盘内肿瘤坏死因子α、白介素1β的影响

摘要

目的 观察脂肪干细胞复合可注射温敏型壳聚糖支架移植对退变早期兔椎间盘内肿瘤坏死因子α(TNF-α)、白介素1β(IL-1β)含量的影响.方法 24只新西兰大白兔,雌雄不限,随机分为髓核抽吸组、脂肪干细胞壳聚糖支架复合移植组、单纯支架移植组以及单纯椎间盘暴露组.术后2、4、8周分别将每组处死2只兔,使用ELISA方法检测L2-3、L3-4、L4-5、L5-6椎间盘内TNF-α、IL-1β含量.结果 24只动物均存活.髓核抽吸组与单纯椎间盘暴露组相比,TNF-α、IL-1β浓度升高(P＜0.05);复合移植组、单纯支架移植组与单纯椎间盘暴露组相比较,TNF-α、IL-1β浓度均降低(P＜0.05);复合移植组与单纯支架移植组相比,8周时IL-1β降低(P＜0.05).结论 脂肪干细胞与温敏型壳聚糖支架在兔椎间盘退变早期能抑制TNF-α、IL-1β表达,可能减轻炎症反应.%Objective To observe the effect of adipose-derived stem cells (ADSCs) compound injective thermo-sensitive chitosan scaffold transplantation on content of tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β) in early degenerate lumbar intervertebral disc of rabbits. Methods 24 white New Zealand rabbits (no limit of male or female) were randomly and equally divided into 4 groups: A. Degeneration model group: nucleus aspiration. B. ADSCs compound chitosan transplantation group. C. Cell-free chitosan transplantation group. D. Blank control group: only explore the target intervertebral disc. When aspirate pulposus with 21G needle, inject ADSCs-scaffold complex and chitosan scaffold respectively. The samples of L2-3, L3-4 , L4-5, L5-6 intervertebral disc were obtained from 2 rabit in each group 2, 4, 8 weeks after operation. The contents of TNF-α, IL-1β were measured with ELISA.Results All animals survived after the operation. Compare with the blank control group, the contents of TNF-α, IL-1β in degeneration model group increased significantly (P＜0. 05). It decreased significantly (P＜0. 05) in ADSCs compound chitosan transplantation group and cell-free chitosan transplantation group compared to model group. IL-1β decreased significantly (P＜0. 05) 8 week after operation in ADSCs compound chitosan transplantation group compared to cell-free chitosan transplantation group. Conclusion -ADSCs compound injective thermo-sensitive chitosan scaffold transplantation in early degenerate lumbar intervertebral disc could decrease the content of TNF-α, IL-1β, and may regulate the inflammatory response.