硫酸镁联合单唾液酸神经节苷脂对脊髓损伤大鼠运动功能恢复的影响

摘要

目的 观察硫酸镁联合单唾液酸神经节苷脂(GM1)对脊髓损伤(SCI)大鼠运动功能恢复的影响.方法 健康成年SD大鼠48只,采用Allen法(10 g×25 mm)在T9造成急性脊髓损伤动物模型.动物随机分为4组:硫酸镁治疗组(A组)、GM1治疗组(B组)、硫酸镁+GM1治疗组(C组)和对照组(D组),每组12只.伤后第1天、第3天和第7天分别用BBB评分和斜板试验观察大鼠运动功能的恢复情况;用硫代巴比妥酸法检测丙二醛(MDA)浓度,观察自由基变化.结果 大鼠脊髓损伤后A、B、C组BBB评分和斜板试验优于对照组,第3天、第7天BBB评分和斜板试验C组优于A、B组(P<0.05).大鼠脊髓损伤后A、B、C组MDA浓度低于对照组(P<0.05),C组明显低于A、B组(P<0.05).结论 大鼠脊髓损伤后早期使用硫酸镁联合GM1对其运动功能的恢复有促进作用,效果优于硫酸镁及GM1单独用药.%Objective To investigate the effect of magnesium sulfate combined with monosialoganglioside on the recovery of motor function after spinal cord injury in rats. Methods 48 healthy adult rats were randomly divided into groups A, B, C and D, and SCI was made by Allen's mocde(10 g×25 mm) on spinal cord T9 extradually, 12 rats in each group. On the 1st d, 3rd d and 7th d after SCI, the recovery of motor function after spinal cord injury in rats was assessed with Basso-Beattie-Bresnahan(BBB)scale and slanting board test. Thiobarbituric acid was used to detect the concentration of malondialdehyde, and was observed the change of free radicals. Results After spinal cord injury in rats, BBB scores and slanting board test of groups A, B and C were better than group D.BBB scores and slanting board test of group C was better than groups A and B, which had significant difference on the 3rd d and 7th d after injury(P＜0. 05). After spinal cord injury in rats, concentration of malondialdehyde of groups A, B and C were lower than group D(P＜0. 05). Concentration of malondialdehyde of group C was lower than groups A and B, which had significant difference after injury(P＜0. 05). Conclusion Magnesium sulfate combined with GM1 can promote the recovery of motor function early after spinal cord injury in rats, and is superior to magnesium sulfate or GM1.