电刺激对大鼠脊髓损伤后神经生长因子表达的影响

摘要

Objective To investigate the effects of electrical stimulation on the expression of nerve growth factor (NGF) in adult rats with spinal cord injury. Methods 72 adult SD rats were randomly divided into 3 groups: damage control group, electrical stimulation group and normal group. The spinal cord incomplete injury model on T9 was made with Allen's method. The electrical stimulation group received electrical stimulation for 7 d. They were assessed with the Basso, Beattie & Bresnahan locomotor rating scale (BBB scale), and the expression of NGF were tested with immunohistochemistry and Western blot 1 d, 3 d, 5 d, 7 d after injury. Results The BBB score improved after spinal cord injury, and electrical stimulation group improved more than control group since the 5th day after injury (P<0.05). The expression of NGF increased in electrical stimulation and injury control group after injury (P<0.05). Conclusion Electrical stimulation after spinal cord injury induces expression of NGF that creates a favorable microenvironment for nerve regeneration.%目的 探讨电刺激对成年大鼠脊髓损伤后脊髓灰质神经元神经生长因子(NGF)表达的影响.方法 健康成年SD大鼠72只,随机分为正常组、损伤对照组、电刺激组.采用Allen法,将后两组复制为脊髓T9损伤模型.术后对电刺激组大鼠进行电刺激治疗7d.于术后1d、3d、5d、7d对3组进行BBB评分,免疫组化和Western blot法检测NGF的表达情况.结果 BBB评分结果显示大鼠脊髓损伤后有自行恢复功能,从第5天开始电刺激组与损伤对照组有显著性差异(P＜0.05).脊髓损伤后,电刺激组和损伤对照组NGF表达均持续升高(P＜0.05),电刺激组表达多于损伤对照组(P＜0.05).结论 脊髓损伤后电刺激诱导损伤区NGF表达,从而创造了有利于神经再生的微环境.