热预处理对超负荷训练大鼠心肌蛋白激酶C表达的影响

摘要

目的：观察热预处理(HP)对超负荷训练大鼠心肌蛋白激酶C (PKC)δ、PKCε表达的影响。方法3月龄雄性Sprague-Dawley大鼠25只分为对照组(n=5)、训练组(n=10)和HP组(n=10)。超负荷训练8周后应用免疫组化法、Real-time PCR和Western blotting检测心肌组织PKCδ、PKCε表达。结果三种检测方法均显示，训练组大鼠心肌PKCδ表达较对照组和HP组增高(P<0.05)。Real-time PCR与免疫组化检测结果显示，训练组PKCε表达低于对照组和HP组(P<0.05)；Western blotting结果显示三组PKCε表达无显著性差异(P>0.05)。结论在热预处理心肌保护效应中，PKC可能发挥了重要的作用。%Objective To investigate the effects of heat preconditioning (HP) on the expression of protein kinase C (PKC)δand PKCεin the myocardium of overload exercise rats. Methods 25 male three-month-old Sprague-Dauley rats were divided into control group (n=5), exercise group (n=10) and HP group (n=10). The expression of PKCδand PKCεin the myocardium was detected with immunohistochemis-try, real-time PCR and Western blotting 8 weeks after overload exercise. Results The expression of PKCδsignificantly increased in the exer-cise group compared with the control group and the HP group (P<0.05). In another hand, PCR and immunohistochemistry showed the ex-pression of PKCεin the exercise group decreased compared with the control group and the HP group (P<0.05), but it was not significantly different among the groups using Western blotting (P>0.05). Conclusion PKCs may play an important role in the HP-induced cardio-protec-tion during overload exercise.