目的：研究莫诺苷对大鼠脑缺血再灌注3 d皮层梗死周边区基质金属蛋白酶(MMP)-2、MMP-9表达的影响。方法健康成年雄性Sprague-Dawley大鼠15只随机分为假手术组(n=3)，模型组(n=3)，莫诺苷小剂量组(n=3)、中剂量组(n=3)及大剂量组(n=3)。采用线栓法制备大鼠大脑中动脉阻塞(MCAO)30 min再灌注模型。术后3 h，莫诺苷各组予莫诺苷30 mg/kg、90 mg/kg、270 mg/kg每天1次灌胃。术后3 d，免疫组化染色观察脑缺血皮层梗死周边区MMP-2、MMP-9表达。结果与假手术组比较，模型组皮层梗死周边区MMP-2、MMP-9表达明显著升高(P<0.01)。与模型组比较，莫诺苷各组MMP-2、MMP-9表达明显降低(P<0.01)。结论莫诺苷能降低大鼠脑缺血再灌注3 d皮层梗死周边区MMP-2、MMP-9的表达，从而保护血脑屏障功能。%Objective To study the effects of morroniside on the expression of matrix metalloproteinase (MMP)-2 and MMP-9 in the peri-infarct cortex 3 days after cerebral ischemia-reperfusion. Methods 15 male Sprague-Dawley rats were randomly divided into sham group (n=3), ischemia group (n=3), and morroniside groups (low, medium and high dosage groups, n=3). The middle cerebral artery were oc-cluded for 30 min, and re-perfused. Morroniside was administered intragastrically once a day at dose of 30 mg/kg, 90 mg/kg and 270 mg/kg 3 hours after operation. The expression of MMP-2 and MMP-9 in peri-infarct cortex were detected with immunohistochemistry staining 3 days after operation. Results The expression of MMP-2 and MMP-9 increased in the ischemia group compared with the sham group (P<0.01), and it decreased in all the morroniside groups compared with the ischemia group (P<0.01). Conclusion Morroniside could decrease the expression of MMP-2 and MMP-9 in the peri-infarct cortex 3 days after ischemia, suggesting protecting the function of blood-brain barri-er from ischemia.
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