The complex of Eu 3+ and Enoxacin（ENX） and Phen was synthesized. Its compositions is Eu（ENX）3（phen）Cl3·2H2O. Its fluorescence spectra were studied. The results show that the strong fluorescence emitting of 592 and 616 nm for Eu 3+ in the Eu（ENX）3（phen）Cl3·2H2O complex is related to the transitions 5D0-7F1 and 5D0-7F2 respectively. A sensitive method for the determination of adenosine triphosphate（ATP） by fluorescence quenching is proposed. The method is based on the ability of ATP to inhibit the formation of a strong fluorescent complex of Eu（ENX）3（phen）Cl3·2H2O. In conditions of pH 6.7 in HAc-NaAc buffer and λ ex=340 nm and λ em=616 nm, the linear range of the determination is 0.4～ 10 μg·ml -1 and the detection limit is 0.034 μg·ml -1 for ATP. The method was applied to determine ATP in adenosine disodium triphosphate injection samples with relative standard deviation of 212% and recovery of 94.8%～1037%.
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