Synthesis and Fluorescence of Eu-Ibuprofenum-Phen Complex and Determination of ATP by Fluorescence Quenching of Complex

摘要

The complex Eu（IB）3（phen）·2H2O was synthesized. The results show that the strong fluorescence emitting of 593 and 615 nm for Eu3+ in the Eu（IB）3（phen） complex is related to the transitions （（）5D0-（）7F1） and （（）5D0-（）7F2,） respectively. A sensitive method for the determination of adenosine disodium triphosphate （ATP） by fluorescence quenching is proposed. The method is based on the ability of ATP to inhibit the formation of a strong fluorescent complex of Eu（IB）3（phen）. In conditions of pH 6.7 HAc-NaAc buffer, λex=273 nm and λem=615 nm, the linear range of the determination was 0.1～10 μg·ml-1 and the detection limit was 0.03 μg·ml-1 for ATP. The method has been applied to determine ATP in adenosine disodium triphosphate tablets samples with relative standard deviation of 2.18% and recovery of （98.4%～）104.0%.