电针干预对放射线照射小鼠海马神经元凋亡的影响及机制研究

摘要

Objective To investigate the effect and mechanism of electroacupuncture at acupoints on radiation-induced hippocampal neuronal apoptosis in C57 mice. Methods Forty one-month-old C 57/6J mice were equally and randomly divided into control group, model group(8 Gy), acupoint-electroacupuncture group, and non-acupoint-electroacupuncture group. In the acupoint-electroacupuncture group,mice received electroacupuncture intervention at Baihui, Fengfu, and Shenshu points. The novel object recognition test was used to evaluate the cognitive, learning, and memory abilities in mice. Immunohistochemistry was used to determine the levels of caspase-3 and apoptosis-inducing factor(AIF). TUNEL assay was used to detect DNA breaks. The data were analyzed by one-way analysis of variance and the Tukey's multiple comparison. Results Compared with the control group, the model group had a significantly reduced recognition index(P= 0.040);compared with the model group, the acupoint-electroacupuncture group had a significantly increased recognition index(P=0.043),shown by significantly longer time spent exploring novel objects(P=0.035).According to the results of immunohistochemistry,the model group had significantly higher apoptosis rate and levels of caspase-3 and AIF in hippocampal neurons than the control group(P= 0.002, 0.003, 0.023), while the acupoint-electroacupuncture group had significantly lower apoptosis rate and levels of caspase-3 and AIF in hippocampal neurons than the model group(P=0.027, 0.005, 0.004). Conclusions Radiation can induce hippocampal neuronal apoptosis and damage cognitive function in mice. Electroacupuncture intervention can, to some degree, reduce above damages.%目的 观察电针穴位对放射线照射C57小鼠海马区神经元细胞凋亡的影响及机制.方法 1个月龄C57BL/6J小鼠随机分成对照组、放射线照射模型组(8Gy)、电针穴位组和电针非穴位组,每组10只.电针穴位组利用电针百会、风府和肾俞穴位分别进行干预治疗.新物体认知实验测试其认知及学习记忆能力.免疫组化方法检测观察Caspase-3、AIF等细胞凋亡相关指标,TUNEL法检测DNA断裂.单因素方差分析结合Tukey's多重比较检验.结果 与对照组比较,模型组小鼠新物体认知指数下降(P=0.040);与模型组比较,电针穴位组小鼠的认知指数显著增加(P=0.043),表现为对新物体OC的探索时间增加(P=0.035).免疫组化结果显示模型组小鼠海马区神经元凋亡阳性百分比、caspase-3和AIF蛋白表达均明显高于对照组(P=0.002、0.003、0.023);而与模型组比较,电针穴位组小鼠海马区神经元细胞凋亡阳性百分比、caspase-3和AIF表达量显著降低(P=0.027、0.005、0.004).结论 放射线照射可诱发小鼠海马区神经元凋亡,损伤小鼠认知功能,电针干预可一定程度改善上述损伤作用.