短串联重复序列基因座Penta D和Penta E与主动攻击行为的关联研究

摘要

Objective To investigate the relationship of the initiative-aggressive behaviors and short tandem repeats (STRs) loci Penta D and Penta E.Methods The biological samples of 307 initiativeaggressive violent offenders and 459 healthy persons without violent behavior were collected.Then all of the sample were amplified by PowerPlex(R) 16 HS System and separated by electrophoresis to compare the genotypes and alleles frequency of Penta D and Penta E in both groups.Results Penta D and Penta E loci in both groups were found to coincide with Hardy-Weinberg equilibrium(P ＞0.05).There were significant difference of alleles and genotypes frequency at loci Penta D and Penta E between both groups (alleles:P =0.000 0,P =0.001 3; genotypes:P =0.001 2; P =0.000 0).Univarite analysis showed significant differences at allelle 5,6 on Penta D and allelle 9 on Penta E between both groups (P =0.002 4,P =0.001 6,P =0.002 1),with the OR values being 0.113 (95％ CI:0.015-0.869),12.092 (95 ％ CI:1.506-96.924),0.225 (95％ CI:0.078-0.648),respectively.Conclusion Penta D and Penta E might be associated with initiative-aggressive behavior,with allelle 6 on Penta D being the susceptible factor and allelle 5 on Penta D and allelle 9 on Penta E might being the protective factors.%目的 探讨主动攻击行为与相关短串联重复序列(short tandem repeats,STR)基因座Penta D、Penta E的关联情况.方法 运用PowerPlex(R) 16 HS System荧光标记复合扩增体系对307例主动攻击行为罪犯(研究组)与459名非攻击行为健康个体(对照组)样本进行PCR复合扩增,然后应用美国ABI3130XL型基因分析系统对扩增产物进行电泳和基因检测,观察2组Penta D、Penta E基因座等位基因及基因型频率的差异.结果 Penta D、Penta E基因座均符合遗传平衡定律(Hardy-Weinberg定律;均P＞0.05);2组Penta D、Penta E基因座等位基因及基因型频率分布差异有统计学意义(等位基因:P=0.000 0,P=0.001 3;基因型:P =0.001 2,P=0.000 0);单因素分析显示,2组在Penta D基因座等位基因5、6和Penta E基因座等位基因9的频率分布差异均有统计学意义(P=0.002 4,P=0.001 6,P=0.002 1),OR值分别为0.113(95％ CI:0.015 ～ 0.869)、12.092(95％ CI:1.506 ～96.924)、0.225 (95％ CI:0.078 ～0.648).结论 Penta D和Penta E基因座多态性与主动攻击行为的发生可能相关联;Penta D基因座的等位基因6可能为主动攻击行为发生的易感因素,PentaD基因座的等位基因5和Penta E基因座的等位基因9可能为主动攻击行为发生的抗性因素.