Objective To discuss the application effects of trinitarian visualization teaching model in endoscopy reprocessing teaching practice. Methods Subjects in the study were divided into the trinitarian visualization teaching group (n=40) and the traditional teaching group (n=43) by random number table method; Compliance and credibility rating of endoscope reprocessing procedures were compared between 2 groups. 2 weeks after training, objectives were examined and assessed by questionnaires separately. Results Compliance of the trinitarian visualization teaching group was higher than the traditional teaching group (χ2=10.501, P=0.033). The trinitarian visualization teaching group had a higher rating in compliance, with 47.47 credibility points, theory test (93.79 ± 3.81) points and 93.02%(40/43) of operation pass. The traditional teaching group had a credibility point of 43.68 credibility points, with theory test (85.25±3.14) points and 77.50%(31/40) of operation pass. Significant difference between the two groups were statistical significant (t=4.84 and 11.097, χ2=4.038,P<0.01 or 0.05). Conclusions The trinitarian visualization teaching model can be used to improve the reprocessing training quality, to save time and human resource and to reach the training demand.%目的：探讨三位一体可视化教学方法在内镜清洗消毒规范化培训实践中的应用效果。方法按照随机数字表法将研究对象分为2组：传统教学组43人和三位一体教学组40人，培训时间为2d。比较2组学员对内镜清洗消毒步骤的依从性及对不同教学方法的公信力评分，同时收集培训2周后学员的理论成绩得分和实践考核合格率。结果三位一体教学组依从性评分高于传统教学组（χ2=10.501，P=0.033）；三位一体教学组公信力评分、培训2周后理论成绩得分和实践考核合格率分别为47.47分、（93.79±3.81）分、93.02%（40/43），传统教学组分别为43.68分、（85.25±3.14）分、77.50%（31/40），差异有统计学意义（t=4.84和11.097、χ2=4.038，P＜0.01或0.05）。结论三位一体可视化教学方法有助于提高内镜清洗消毒规范化培训质量，节约人力和时间，更加符合消化内镜清洗消毒规范化培训的需求，值得临床推广和应用。
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