慢性荨麻疹患者外周血单一核细胞T-bet/GATA-3的表达研究

摘要

目的 检测慢性荨麻疹患者外周血单-核细胞(PBMC)T-bet、GATA-3 mRNA的表达,计算T-bet/GATA-3比值,探讨Th1/Th2失衡与慢性荨麻疹的关系.方法 采用实时定量多聚合酶链反应(RT-PCR)方法检测30例慢性荨麻疹患者(CU)和30例健康对照者PBMC中T-bet和GATA-3 mRNA的表达水平,并计算T-bet/GATA-3比值.结果 CU组PBMC中T-bet mRNA表达水平较对照组降低,而GATA-3 mRNA的水平较对照组升高,CU组T-bet/GATA-3比值较对照组显著下降.结论 慢性荨麻疹患者T-bet/GATA-3表达异常,患者存在显著的Th1/Th2免疫失衡.%Objective To detect the T-bet and GATA-3 expression in chronic urticaria patients,and calculate the ratio of T-bet/GATA-3,then to investigate the relationship between the immune-imbalance and chronic urticaria.Methods Real-time quantitative PCR technology was used to detect the expression of T-bet and GATA-3 in peripheral blood mononuclear ceils from 30 chronic urticaria patients(CU group)and 30 normal in dividuals(control group).And then the ratio of T-bet / GATA-3 was calculated and compared.Results Tbet expression in CU group was lower than control group(P =0.0001),while GATA-3 was higher than that in control group(P =0.0001),and the ratio of T-bet / GATA-3 in CU group was obviously lower than that in control group (P =0.0001).Conclusions T-bet / GATA-3 decreased in chronic urticaria patients,there is prominent immune imbalance of Th1/Th2 in the chronic urticaria.