人参皂甙肠道代谢物Compound K对人胃癌细胞增殖及凋亡相关基因表达的影响

摘要

Objective To investigate the effect of CK on proliferation,apoptosis of BGC823 cells.Methods BGC823 cells were treated with CK at different concentrations,cell viability was assayed using MTT methods.Cell cycle,apoptosis rate were assayed by flow cytometry.The expression of apoptosis-related protein were detected by Western blotting.Results After BGC823 cells were treated with 5 μmol/L CK for 48 h,the growth cycle was arrested on G2/M phase,the apoptosis rate was (21.17 ± 3.16)％.The expressions of Bcl-2,Bid protein was down-regulated after CK treatment,while the Fas and Fas-L protein were not significantly changed.Conclusions CK inhibits proliferation of BGC823 cells,and induces apoptosis via mitochondrion-mediated pathway.%目的 观察人参皂甙肠道代谢物Compound K (CK)对人胃癌细胞BGC823细胞增殖、凋亡的影响.方法 实验分组:对照组(PBS溶液),实验组(浓度分别为2.5、5、7.5、10 μmol/L).噻唑蓝(MTT)比色法分别检测CK作用于细胞后其活力及生长抑制率;流式细胞术检测CK作用后细胞周期时相、凋亡率;免疫印迹法(Western blot)检测凋亡相关蛋白的表达.结果 5μmol/L CK作用细胞48 h时,细胞生长周期阻滞于G2/M期,细胞凋亡率为(21.17±3.16)％;Western blot检测显示Bcl-2,Bid蛋白表达随着药物浓度的增加而减少,Fas和Fas-L蛋白表达量却没有明显变化.结论 CK是通过线粒体介导的凋亡通路途径来诱导人胃癌细胞发生凋亡的.