Objective To characterize the changes of the viscoelasticity of fibroblasts derived from uterosacral ligament of pelvic organ prolapse and nomal ones. Methods 11 patients with POP and 13 patients without POP who underwent hysterectomy surgery in the Obstetric and Gynecological Department of the Second Hospital of Hebei Medical University between December 2009 and June 2010 were recruited in this study, fibroblasts of experimental group (n = 18) and control group ( n = 22 ) were observed respectively. Uterosacral ligament fibroblasts of both groups were cultured by tissue inoculation in vitro. The cultured cells were identified by morphology with light microscope and the cellscertain antigens were detected with immunocytochemical method. This study utilized the micropipette aspiration technique with viscoelastic solid modal to investigate the viscoelastic properties of the fibroblasts of both groups. Results The cells showed shuttle shape and expressed vimentin which confirmed the fibroblasts'phenotype. The viscoelastic properties of the Uterosacral ligament fibroblasts in POP group exhibited a significantly lower equilibrium modulus E? .instantaneous modulus E0 and apparent viscosity (μ [E∞=(124. 62 ±20. 22) Pa], [E0 = (429. 25 ± 105. 89)Pa] , [μ= (718. 53 ± 159. 08)Pa ·s]compared with control group[E∞=( 170. 17 ±24. 65) Pa, E0 = (495. 26 ±69. 39) Pa] ,[ μ= (845. 35 ± 155. 34) Pa · s], P < 0. 05. Conclusions The changes in the state of cytoskeleton structure and function of the fibroblasts maybe the key link of pelvic organ prolapse.%目的 探讨盆腔器官脱垂(pelvic organ prolapse,POP)患者骶韧带成纤维细胞粘弹性变化.方法 选取2009年12月至2010年6月河北医科大学第二医院妇产科POP患者11例为观察组,同期非POP患者13例为对照组,取骶韧带进行成纤维细胞的原代培养.光镜观察与免疫细胞化学染色法进行成纤维细胞鉴定,取1代的成纤维细胞(实验组观察细胞数为18,对照组观察细胞数为22),采用微管吸吮技术表征骶韧带成纤维细胞的粘弹性特性.结果 培养的细胞呈梭形,表达波形蛋白(vimentin),POP患者骶韧带成纤维细胞粘弹特性明显低于正常对照组,病例组骶韧带成纤维细胞平衡模量[E∞=(124.62±20.22)Pa]、瞬间模量[E0=(429.25±105.89)Pa]、黏性系数[μ=(718.53±159.08)Pa·s]均显著低于对照组[E∞=(170.17±24.65)Pa]、[E0=(495.26±69.39)Pa、μ=(845.35±155.34)Pa·s],P＜0.05.结论 POP患者成纤维细胞骨架结构和机 能状态的改变可能是POP发生的关键环节.
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