脂肪组织提取物促进组织工程室内脂肪组织再生的实验研究

摘要

Objective To evaluate the influence of adipose tissue extract on inducing angiogenesis and adipogenesis in adipose tissue engineering chamber in vivo.Methods 6 months' healthy New Zealand rabbits(n =64) were picked.The inguinal fat pads were cultured,centrifuged,filtered,and the liquid was called adipose tissue extract (ATE).Two adipose tissue engineering chamber were built in the rabbit' s back.A week later,0.2 ml normal saline (control group,left) and 0.2 ml ATE (experimental group,right) was respectively injected into the chamber.The contents were evaluated morphometrically,histologically and immunohistochemically 3 days,1 week,2 weeks,3 weeks,4 weeks and 7 weeks after injection.8 rabbits were observed each time.The data regarding the number of the volume of fat flap and blood capillary at each time point were analyzed by paired t test.Results After injection,new tissue volume was significantly increased in the experimental group[(5.12 ± 0.22) ml],compared with that in control group [(4.90 ± 0.15) ml].Early angiogenesis was also increased after ATE injection and the total number of capillaries reached peak 1 week after injection,which was (72.80 ± 9.67) in experimental group and (51.40 ± 6.09) in control group.In the mid-term of experimental period,earlier adipogenesis appeared in experimental group.In the later period,the outer capsule of the new construction was thinner in experimental group which reduced the suppression of the adipogenesis.Conclusions ATE can promote the angiogenesis and adipogenesis in the chamber,and reduce the capsule contracturing,so as to induce the large volume of adipose tissue regeneration.%目的 初步探讨组织工程室模型中脂肪组织提取物对血管和脂肪新生诱导的影响.方法 健康6个月龄新西兰大白兔64只,取兔腹股沟脂肪垫,经培养、离心、过滤后得到脂肪组织提取物(adipose tissue extract,ATE),应用组织工程室技术在兔背部左、右则建立2个组织工程室模型.1周后根据工程室内是否加入ATE分为2组:实验组为兔背部右侧工程室加入0.2 ml ATE,对照组为左侧加入等量生理盐水,注入后3d和1、2、3、4、7周取出标本,每个时间点8只,观察大体形态、体积,行HE染色,CD31检测.采用SPSS 13.0统计软件对每个时间点左、右两侧脂肪瓣的体积及毛细血管数目行配对￡检验.结果 注入ATE后,实验组新生组织体积为(5.12±0.22) ml,较对照组的(4.90±0.15) ml明显增大,早期血管新生和数量明显增多,注入ATE后l周总的毛细血管的数量最高,并且实验组[(72.80±9.67)个]较对照组[(51.40±6.09)]个多,中期新生组织边缘更早出现血管周围的脂肪新生,后期新生组织最外层包膜厚度更薄,减少对内部脂肪新生的抑制,差异均有统计学意义(p＜0.05).结论 ATE能促进组织工程室中脂肪和血管新生,同时减轻包膜挛缩对组织新生的抑制,诱导大体积脂肪组织再生.