昆参颗粒对裸鼠人胃癌模型VEGF-C、VEGFR-3表达的影响

摘要

Objective To probe the effect of Kunshen Granule on the expression of vascular endothelial growth factor-C(VFGF-C) and vascular endothelial growth factor receptor-3(VFGFR-3).Methods 40 BALB/C nude mice were randomly divided into 4 groups: negative control group, 5-Fu group, Kunshen large dose group and small dose group.The nude mice except the negative group were implanted human cancer cell SGC-7901 on stomach near the greater curvature side by means of plastering "OB" gum.And then nude mice were treated with normal saline,5-Fu, Kunshen large dose and small dose respectively.After six weeks, the animals were anesthetized.sacrificed and the local growth transplanted tumor were observed.The expressions of VEGF-C and VEGFR-3 in the local transplanted tumor tissues were detected and analyzed semi-quantitatively by means of Envision immunohistochemistry staining.Results ①The transplanted tumors in 40 nude mice were growing well, and some of them had enlarged abdominal lymph nodes, lymph nodes metastasis rates of 5-Fu group, Kunshen large dose group and small dose group were 50％,40％,60％, significantly lower than negative control group, which was 100％.②Compared to negative control group, the expressions of VEGF-C and VEGFR-3 in 5-Fu group, Kunshen large dose group and small dose group were significandy decreased, the difference had a statistical significance (P ＜0.01).Difference of the expression of VEGF-C between the Kunshen large dose group and 5-Fu group had a statistical significance (P ＜0.05).Difference of the expression of VEGF-C and VEGFR-3 between the Kunshen large dose group and Kunshen small dose group had a statistical significance(P＜0.01).③There is linear correlativity between the expression of VEGF-C, VEGFR-3 and the lymph nodes metastasis nearly(r=0.825, P=0.00).Conclusion Kunshen granule can decreased the expression of the VEGF-C and VEGFR-3, which may be one of the mechanisms inhibiting carcinoma lymph nodes metastasis.%目的 探讨昆参颗粒对裸鼠人胃癌模型血管内皮生长因子C(VEGF-C)、血管内皮生长因子受体-3(VEGFR-3)的影响.方法 将40只BALB/C无胸腺裸鼠随机分为4组:阴性对照组、5-Fu组、昆参高剂量组、昆参低剂量组,每组10只.采用"OB"胶粘贴法建立裸鼠人胃癌SGC-7901原位移植模型,术后48小时开始分别给予生理盐水、5-Fu、昆参高剂量、昆参低剂量治疗,6周后观察裸鼠胃癌淋巴结转移情况,用Envision免疫组化染色法检测原位移植瘤组织中VEGF-C、VEGFR-3蛋白的表达情况.结果 ①40只裸鼠胃癌原位移植瘤均有生长,部分伴有淋巴结转移,阴性对照组、5-Fu组、昆参高剂量组、昆参低剂量组的淋巴结转移率分别为100%、50%、40%、60%.②药物干预的三组胃癌组织中VEGF-C、VEGFR-3的表达明显少于阴性对照组,有统计学差异(P<0.01).昆参高剂量组VEGF-C表达明显少于5-Fu组,有统计学差异(P<0.05).昆参高剂量组比昆参低剂量组VEGF-C、VEGFR-3的表达减少,有统计学差异(P<0.01).③VEGF-C、VEGFR-3、淋巴结转移之间存在密切直线相关性(r=0.825,P=0.00).结论 昆参颗粒能明显抑制VEGF-C、VEGFR-3蛋白的表达,可能是昆参颗粒抑制胃癌淋巴结转移的重要作用机制.