雷公藤片和雷公藤多苷片抗炎作用量效研究

摘要

Objective To explore the dose range of anti-inflammatory effect of Tripterygium wilfordii tablets (TWT) and Tripterygium wilfordii polyglycoside (TWP)tablets and compare with the results of them, so as to provide the basis for clinical medication. Methods Mice were respectively given the different doses of TWT and TWP for 3 consecutive days by gavage. The anti-inflammatory effects of TWT and TWP were explored according to the model of ear edema by mixture of compound croton oil we made. ED50 and ED95 of anti-inflammatory efficacy and LD50 and LD5 of acute toxicity test were calculated. Results ED50 and 95% confidence limit of TWT and TWP were respectively 17.753 (13.567~21.448)μg·kg-1, 9.236 (5.570~11.915) mg·kg-1, which were equal to triptolide of 17.753μg·kg-1, 8.922μg·kg-1. They were respectively equal to 20.30 and 10.21 times of 70 kg person's daily dried medicine expenses. Conclusion TWT and TWP show good suppression on the acute inflammation caused by mixture of compound croton oil. The potency of TWP is better than that of TWT. Triptolide with good effect of anti-inflammation shows a good"dose-effect"relationship. But it is not the only material basis of Tripterygium wilfordii for anti-inflammatory effect. The effective dose ranges of them show a certain dose-effect relationship. The mechanism of anti-inflammatory effect needs a further study.%目的明确雷公藤片和雷公藤多苷片发挥抗炎作用的剂量范围，比较其作用效果异同，为临床用药提供依据。方法给小鼠灌胃不同量的雷公藤片和雷公藤多苷片，连续3天，通过巴豆油混合致炎液致小鼠耳肿胀模型计算雷公藤片和雷公藤多苷片对抗急性炎症的半数有效量（ED50），探讨雷公藤片和雷公藤多苷片对小鼠急性炎症的抑制作用差异。结果雷公藤片和雷公藤多苷片发挥抗炎作用的ED50分别为17.753μg·kg-1和9.236 mg·kg-1，折合成雷公藤甲素分别为17.753和8.922μg·kg-1，分别相当于临床70 kg人临床日用量的20.30倍和10.21倍。结论雷公藤片、雷公藤多苷片均可拮抗巴豆油混合致炎液导致的急性炎症，且呈现良好的量效关系。抗炎作用强度为雷公藤多苷片>雷公藤片。提示雷公藤甲素、雷公藤多苷均是雷公藤抗炎作用的物质基础。雷公藤抗炎作用机制有待于进一步研究。