制首乌联合定量运动对大鼠肝脏微循环及肝功能的影响

摘要

目的：探讨长期服用制首乌联合定量运动对肝脏微循环和肝功能的影响。方法采用2×2析因设计，大鼠随机分为静止不给药（S/C）组，静止给药（S/D）组，游泳不给药（SW/C）组，游泳给药（SW/D）组，每天给予不同水平的制首乌醇提物（0g·kg-1、20g·kg-1 p.o）及不同水平的游泳运动（0h、1h），连续30d。采用激光散斑血流监测视频系统检测大鼠肝脏微循环血流灌注量的变化；检测大鼠血清NOS3含量及肝功能9项生化指标。结果与S/C组比，制首乌醇提物降低大鼠肝脏血流平均灌注量（P<0.01），同时显著降低血清内皮源一氧化氮合成酶（NOS3）含量（P<0.05），并可显著升高大鼠总胆红素（TBIL）、直接胆红素（DBIL），显著降低间接胆红素（IBIL）（均P<0.001）。游泳对制首乌引起的肝脏微循环灌注量和血清NOS3含量下降均有显著恢复作用，但游泳不能改善制首乌引起的血清胆红素异常。结论本研究首次证明大剂量长期服用制首乌可导致肝脏微循环灌注量障碍，而适当的运动则可能拮抗药源性肝脏微循环障碍。%Objective To investigate the long-term effects of taking Radix Polygoni multiflori Preparata combined quantitative exercise on hepatic microcirculation and liver function. Methods 2 ×2 factor experimental design was used. Rats were randomly divided into four groups, namely, static without drug group (S/C), static with drug group (S/D), swim without drug group(SW/C), swim with drug group(SW/D), given the different levels of Radix Polygoni multiflori Preparata alcohol extract (0g·kg-1, 20g·kg-1 p.o) and different levels of swimming(0h, 1h). After 30 days, using Laser Doppler blood flow imaging system to monitor the change of rat hepatic microcirculation blood flow;the serums of rats were collected to determine the content of NOS3 and the biochemical indexes of liver function. Results Compared with S/C group, Radix Polygoni multiflori Preparata alcohol extract significantly reduced the mean perfusion unit of hepatic microcirculation and serum endothelial nitric oxide synthase(NOS3) content(P<0.05) in rats, while significantly elevated serum total bilirubin(TBIL), direct bilirubin(DBIL), reduced indirect bilirubin(IBIL) (P <0.001). The descent of hepatic microcirculation perfusion unit and serum NOS3 content caused by Radix Polygoni multiflori Preparata can be recovered by swiming, but swimming can not improve the abnormal of serum bilirubin. Conclusion This is the pilot study which demonstrates that hepatic microcirculation perfusion disturbance might be used as one early indicator of liver injury induced by high dose of alcohol extract of Radix Polygoni multiflori Preparata, and appropriate exercise may antagonize the effects by influencing the serum NOS3.