金鸡毛草水提取液急性毒性实验研究

摘要

目的 探讨金鸡毛草水提取液的安全性,为进一步的研究与开发提供理论依据.方法 制备金鸡毛草水提取液,采用经典急性毒性实验方法测定半数致死量(LD50)、最大耐受量(MTD).用不同浓度金鸡毛草水提取液对5组昆明小鼠行预实验测定其半数致死量(LD50),观察小鼠存活率.用金鸡毛草水提取液最大浓度及灌胃体积24 h内连续3次对实验组小鼠灌胃给药,同时予以等体积蒸馏水灌胃对照组,分别观察实验组、对照组给药后小鼠毒性症状及体重变化;14d后采血检测血液生化指标;并处死小鼠进行大体解剖,肉眼观察各脏器变化;取小鼠肝脏及肾脏进行病理组织学检查;计算最大耐受量(MTD).结果 预实验结果未测出小鼠LD50,MTD＞120 g·kg-1;对照组及实验组小鼠体重变化差异无统计学意义(P＜ 0.05),ALT、AST、ALP、Urea、Cr、TP、ALB、Glu、T-Bil各项指标未见明显异常;对照组与实验组小鼠肝脏、肾脏病理未见明显病变.结论 金鸡毛草水提取液对昆明小鼠无急性毒性.%Objective To investigate the safety of aqueous extracts from Gynura divaricata,so as to provide a theoretical foundation for further research and development.Methods Preparing the aqueous extracts from Gynura divaricata,the LD50,MTD were tested by the classical methods of acute toxicity.Five groups of Kunming mice were used for pre-experiment by different concentrations of aqueous extracts from Gynura divaricata,the survival rate of mice were observed,so as to determine the LD50.Mice were given relevant drugs intragastrically every 4 h in 24 h in experimental group,at the same times the blank control group mice were given constant distilled water,and the general symptom,death and weight of mice were observed.Mice were sacrificed 14 days later,and blood was detected,and organs were observed by eyes,the liver and kidney were examined histopathology,and MTD was counted.Results The LD50 of mice was not detected by the pre-experiment,the MTD of acute toxicity test was 120 g·kg-1 The mice's weight change was not significantly different between the control group and the experimental group (P ＜ 0.05).ALT,AST,ALP,Urea,Cr,TP,ALB,Glu and T-Bil of mice were showed no obvious abnormalities.The pathological changes of liver and kidney were not obvious in the control group and the control group.Conclusion Aqueous extracts from Gynura aivaricata had no obvious toxicity for Kunming mice.