大黄素辅助治疗糖尿病肾病的作用研究

摘要

Objective To investigate the auxiliary therapeutic effects and mechanism of emodin on diabetic nephropathy (DN). Methods DN rats were induced with GK rats fed with high fat diet. Forty DN rats were divided into 5 groups: model group, repaglinide (0.7 mg·kg-1) group, emodin (20, 40 and 80 mg·kg-1) plus repaglinide (0.7 mg·kg-1) group according to the level of blood glucose. Each group consists of 8 rats. Animals were administered orally twice daily for continuous sixty days. Hemorheology index, blood glucose(GLU), blood fat(TC、TG), inflammatory cytokines(IL-6、TNF-α) and renal function index (BUN、CR、mAlb) were determined after the last intragastric administration, and histopathological changes of kidney were observed by HE staining. Results Compared with repaglinide group, the level of TC, TG, BUN, CR, IL-6, TNF-α, mAlb and hemorheology index were decreased, at the same time pathological changes of kidney were alleviated significantly in emodin (40 and 80 mg·kg-1) plus repaglinide (0.7 mg·kg-1) groups. Conclusion Emodin (40 and 80 mg·kg-1) has a remarkable renal protective effects on kidney of DN rats.%目的 探讨大黄素对糖尿病肾病有无辅助治疗作用及作用机理.方法 给予GK大鼠高脂饲料制备40只DN大鼠模型,按照血糖高低分为模型组、瑞格列奈0.7 mg·kg-1对照组、瑞格列奈0.7 mg·kg-1+大黄素(20、40、80 mg·kg-1)三个剂量组,每组8只大鼠.动物每天灌胃给药2次,连续60天.末次给药后检测血液流变学指标、血糖(GLU)、血脂(TC、TG)、炎细胞因子(IL-6、TNF-α)、肾功能指标(BUN、CR、mAlb)和HE染色观察肾脏病理组织学改变.结果 与瑞格列奈组比较,瑞格列奈0.7 mg·kg-1+大黄素(40、80 mg·kg-1)组大鼠血浆中TC、TG、BUN、CR、IL-6、TNF-α 和mAlb以及血黏度明显降低,肾脏病变明显减轻.结论 应用降糖药同时给予大黄素可明显保护DN模型大鼠的肾脏.