To investigate the anti-metastasis effect of emodin on the pancreatic cancer in vitro and in vivo. Human pancreatic cancer cell line SW1980 was treated with different concentrations of emodin (10, 30, 40 μmol·L~(-1)) for 2 h, the effects of emodin on migration and invasion of SW1980 cells were examined by using wound assay. Western blot was used to detect the protein expression of NF-k and MMP-9 in SW1980 cells after various concentrations of emodin (10, 30, 40 μmol.L~(-1)) treatment for 48 h. Eight weeks after implantation, the presences of metastasis were evaluated respectively after the mice were sacrificed. Immuno-histochemistry was used to detect the positive expression of CD41, NF_(-k)B and MMP-9 in the tumors. Emodin suppressed the migration and invasion of SW1980 cells in a dose-dependent manner. Western bolt assay indicated that emodin downregulated the expression of NF-k and MMP-9 proteins. The incidences of metastasis were de-creased significantly in L-EMO group and H-EMO group as compared with that in control group. The percentage of CD41, NF_(_k)and MMP-9-positive cells in the tumors were significantly reduced by the administration of emodin.
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