cqvip:The ability of soybean lipoxygenase to mediate the N-demethylation of imipramine and related drugs in the presence of hydrogen peroxide was examined.Under optimal assay conditions,Vmax values of 14 to 18 mol formaldehyde·min^-1·mol^-1 enzyme were observed.An inhibition of formaldehyde and desipramine formation by nordihydroguaiaretic acid confirmed the lipoxygenase involvement.The blockade of the reaction by glutathione,dithiothreitol butylated hydroxyanisole (BHA) and butylated hydroxytoluene(BHT) indicated the generation of a free radical intermediate from imipramine.Desipramine,trimipramine,clomipramine,and diltiazem,but not amitriptyline and doxepin,were also oxidized,albeit at a lower rate.Collectively,the evidence gathered in this study suggests,for the first time,that tricyclic antidepressant drugs may undergo lipoxygenase-catalyzed N-demethylation.
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机译：La rencontre analytique dans les dispositifs à mediations thérapeutiques, aux limites de l’analyse↓O encontro analítico nos dispositivos das media??es terapêuticas, nos limites de uma análise↓El encuentro analítico en los dispositivos de las mediaciones terapéuticas, en los confines de un analisis↓Die analytische Begegnung in Systemen zur therapeutischen Mediation, an den Grenzen einer Analyse↓用精神分析法对儿童精神病进行干预性治疗和精神分析法的局限性
