五氯联苯PCB118对人肝癌细胞BEL-7402细胞黏附的影响

摘要

OBJECTIVE To investigate the effects and mechanism of PCB118 on cell-matrix and cel-cel adhesion in human hepatocel ular carcinoma cel s. METHODS Human hepatocel ular carcinoma cel s BEL-7402 were treated with PCB118 0.1,1.0 and 10.0 nmol · L-1 for 4 or 6 d,respectively. Then the cell-matrix adhesion assay and cell aggregation experiments were conducted to study the effect of PCB118 on cell-matrix adhesion and cell-cell adhesion in BEL-7402 cells. Quantitative real-time PCR and Western blotting methods were employed to assess the expression of key cytokines CD29,N-cadherin and E-cadherin. RESULTS The results showed that the cell-matrix adhesion ability of human hepato⁃cellular carcinoma cells BEL-7402 were significantly increased(P<0.05)after treatment with PCB118 0.1,1.0 and 10.0 nmol·L-1 for 6 d,whereas the cell-cell adhesion ability was significantly reduced(P<0.05). Exposure to PCB118(0.1,1.0 and 10.0 nmol·L-1)for 6 d induced significant upregulation of the mRNA expression levels of CD29 and N-cadherin along with the downregulation of E-cadherin(P<0.05). Western blotting analysis revealed that PCB118 exposure significantly increased protein expressions of CD29 and N-cadherin but reduced E-cadherin protein level(P<0.01). CONCLUSION PCB118 exposure affects the expression of CD29,N-cadherin and E-cadherin, which may be involved in PCB118-induced alteration of cell adhesion of hepatocellular carcinoma cell line BEL-7402.%目的：探讨五氯联苯PCB118对人肝癌细胞细胞-基质间和细胞-细胞间黏附的影响和机制。方法人肝癌细胞BEL-7402用PCB1180.1，1.0和10.0 nmol· L-1分别处理4和6 d后，采用细胞-基质间黏附实验检测细胞-基质间黏附，细胞聚集实验检测细胞间黏附，实时荧光定量PCR法和Western蛋白印迹法检测细胞黏附关键因子CD29、E-钙黏着蛋白和N-钙黏着蛋白的表达。结果与细胞对照组相比，人肝癌细胞BEL-7402在PCB1180.1，1.0和10.0 nmol· L-1处理6 d后，细胞-基质间黏附能力明显提高（P<0.05），细胞-细胞间黏附明显降低（P<0.05）。实时荧光定量PCR结果显示，PCB118明显提高黏附关键因子CD29和N-钙黏着蛋白mRNA水平（P<0.05），显著降低E-钙黏着蛋白mRNA水平（P<0.05）。Western蛋白印迹结果表明，PCB118处理后，CD29和N-钙黏着蛋白的蛋白表达显著升高（P<0.01），E-钙黏着蛋白的蛋白表达显著降低（P<0.01）。结论 PCB118影响肝癌细胞黏附并改变黏附因子CD29、E-钙黏着蛋白和N-钙黏着蛋白的表达，这可能与PCB118促进肝癌的发展有关。