反义寡核苷酸抑制缺氧／再给氧时内皮细胞细胞间粘附分子-1的表达

摘要

目的：探讨反义寡核苷酸(As-0DN)对缺氧／再给氧(H／R)时内皮细胞细胞间粘附分子-1(ICAM-1)表达的影响。方法：流式细胞仪测定肾小球血管内皮细胞在缺氧、再给氧及加入AS-0DN后ICAM-1表达的阳性百分率。结果：缺氧10 h，肾小球血管内皮细胞ICAM-1的表达与对照组无显著性差异，再给氧6 h ICAM-1的表达明显高于正常，加入As-ODN后，ICAM-1阳性细胞的百分率下降40.6％。结论：AS-ODN可以降低H/R时内皮细胞ICAM-1的表达。%AIM: To investigate the effect of antisense oligodeoxynucleotides (AS - ODN) on the intercellsular adhesion molecule- 1 (ICAM- 1 ) expression on endothelial cells in hypoxia/reoxygenation(H/R). METHODS: With cultured glomerular vascular endothelial cell in H/R, the positive percentage of ICAM - 1 expression was measured by flow cytometry before and after giving AS - ODN. RESULTS: The ICAM - 1 expression did not increase on glomerular vascular endothelial cell in 10 hours hypoxia compared to control group, it increased in 6 hours reoxygenation, and decreased by 40.6 % after giving AS- OND. CONCLUSION: AS - ODN may decrease the expression of ICAM- 1 on endothelial cells in H/R.