目的:探讨糖皮质激素吸入对哮喘患儿外周血CD4~+CD25~+ 调节性T细胞(Tr)水平的影响.方法:采用糖皮质激素吸入治疗70例发作期哮喘患儿,应用流式细胞术检测患儿外周血的Tr细胞数.结果:糖皮质激素规则治疗后患儿外周血CD4~+CD25~+Tr水平(7.05%±1.61%)明显高于治疗前(5.62%±1.29%),P<0.01.完全控制组患儿外周血CD4~+CD25~+Tr水平最高(7.56%±1.88%),部分控制组患儿次之(7.09%±1.23%),控制不佳组患儿最低(6.11%±1.96%),差异均显著,P<0.05.经激素规则治疗的患儿外周血Tr水平(7.05%±1.61%)明显高于不规则治疗组患儿(5.91%±1.76%),P<0.01.结论:规则使用糖皮质激素吸入疗法可明显提高哮喘患儿外周血Tr水平,Tr水平与哮喘的激素治疗效果有关.%AIM: To investigate the effect of glucocorticoid inhalation on the levels of CD4~+CD25~+ regulatory T cells in peripheral blood of asthmatic children. METHODS: Glucocorticoid inhalator was inhaled by 70 children with attack asthma. The levels of CD4~+CD25~+ Tr in peripheral blood of asthmatic children were tested by flow cytometry (FCM). RESULTS: The CD4~+CD25~+ Tr levels in peripheral blood of asthmatic children were (5.62% ± 1.29% ) and (7.05% ± 1.61%) before and after of regulated glucocorticoid inhalation, respectively (P<0.01). The Tr levels were (7.56% ± 1.88% ) , (7.09% ± 1.23% ) and (6.11% ± 1.96% ) in the complete control group, part control group and poor control group, respectively ( P < 0.05 ). The Tr level in formal treatment group (7.05% ±1.61%) was higher than that in irregular treatment group ( 5.91 % ± 1.76% ), P < 0.01.CONCLUSION: The level of CD4~+CD25~+ Tr is remarkable increased by regulated glucocorticoid inhalation, and the level of Tr can reflect the effects of glucocorticoid inhalation.
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