目的:研究人白血病Reh、HL-60与K562细胞系RUNX3基因P2启动子甲基化状态及RUNX3基因的表达,分析P2启动子甲基化与基因表达之间的关系.方法:运用甲基化特异性PCR(MSP)和RT-PCR检测Reh、HL-60与K562细胞系RUNX3基因P2启动子的甲基化状态及RUNX3基因的表达.结果:Reh及K562细胞系RUNX3基因P2启动子甲基化特异性扩增呈阳性,非甲基化特异性扩增呈阴性,RT-PCR扩增呈阴性;而HL-60细胞系相反,甲基化特异性扩增呈阴性,而非甲基化特异性扩增呈阳性,RT-PCR扩增阳性.结论:Reh及K562细胞中存在RUNX3基因P2启动子的甲基化,且在不同类型白血病细胞系中的甲基化状态不同.%AIM: To investigate the relationship between the methylation status of RUNX3 gene P2 promoter and expression of RUNX3 in human leukemic cell lines Reh, HL - 60 and K562. METHODS: Methylation status of RUNX3 gene P2 promoter was detected by methylation - specific polymerase chain reaction ( MSP ) and the mRNA expression of RUNX3 was determined by RT - PCR in leukemic cell lines Reh, HL-60 and K562. RESULTS: Methylation -specific PCR products were detected in Reh cells and K562 cells, while those in HL - 60 cells were negative. Unmethyla-tion - specific PCR products were only detected in HL - 60 cells. RT - PCR amplification of RUNX3 was negative in Reh cells and K562 cells, but that was positive in HL-60 cells. CONCLUSION: Methylation in P2 promoter of RUNX3 gene exists in human leukemic Reh cells and K562 cells. There is different methylation status in different types of leukemic cell lines.
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