二氢杨梅素联合丝裂霉素对胃癌细胞的生长抑制作用

摘要

目的:探讨二氢杨梅素(AMP)联合细胞化疗药物丝裂霉素(MMC)对胃癌细胞SGC-7901的抑制作用.方法:体外培养人胃癌细胞株SGC-7901,分别设空白对照组、AMP组、MMC组以及AMP+MMC组.用MTT法观察细胞生长;流式细胞术分析细胞凋亡率;Western blotting检测细胞Bcl-2及survivin的表达情况.结果:在AMP组中,当浓度在2.2 mg/L-14.84 mg/L时,AMP对胃癌细胞生长均有抑制作用,以14.84 mg/L抑制作用最明显(60.85%±1.13%,P<0.05).MMC随着浓度由1×10-3g/L增加至1×10g/L,其抑制率也由17.40%±0.30%增加至72.23%±1.36%,呈递增趋势.AMP联合MMC的抑制作用随着MMC浓度由1×10-3 g/L增加至5×10-3g/L,抑制率也由21.83%±2.50%增加至46.70%±1.45%.联合治疗组优于单独给药组.AMP、MMC及AMP+MMC均可以下调Bcl-2及survivin的表达,其中AMP+MMC组下调作用最明显.结论:AMP联合MMC可以增强对胃癌细胞的抑制作用,联合应用可以减低MMC的剂量;其抑制肿瘤作用可能与下调Bcl-2及survivin蛋白的表达有关.%AIM: To investigate the synergistic effects of ampelopsin ( AMP ) and a chemotherapeutic drug mitomycin ( MMC ) on the proliferation of gastric cancer cell line SGC -7901.METHODS: SGC -7901 cells were cultured in vitro and divided into 4 groups: control group, AMP group, MMC group and AMP + MMC group.Cell proliferation was measured by MTT method.The apoptotic index was examined by flow cytometry.The expression of apoptotic proteins, Bcl- 2 and survivin, was detected by Western blotting.RESULTS: AMP at the concentrations ranging from 2.2 mg/L to 14.84 mg/L exerted inhibitory effect on the growth of SGC - 7901 cells.Cell proliferation in AMP ( 14.84 mg/L ) group was inhibitory by ( 60.85 ± 1.13 ) ％ , significantly higher than that in control group ( P ＜0.05 ).The inhibitory rates of cell proliferation varied from ( 17.40 ±0.30 ) ％ to ( 72.23 ± 1.36 ) ％ when the concentrations of MMC increased from 1× 10-3 g/L to 1 × 10-2 g/L.AMP combined with MMC showed a synergistic effect on the growth of SGC -7901 cells.The inhibitory rates of cell proliferation varied from ( 21.83 ± 2.50 ) ％ to ( 46.70 ± 1.45 ) ％ when the concentrations of MMC increased from 1 × 10 -3g/L to 5 × 10-3 g/L.The inhibitory effect of AMP plus MMC was higher than that of MMC or AMP alone.The protein levels of Bcl - 2 and survivin were inhibited in AMP group, MMC group and AMP + MMC group, and were significantly lower in AMP + MMC group than those in AMP group or MMC group.CONCLUSION: AMP enhances the inhibitory effect of MMC on the growth of SGC -7901 cells.The mechanism is related to the inhibition of apoptotic protein expression.