AIM : To compare the effects of B50, a mono - carbonyl analogue of curcumin, on the proliferation and apoptosis between homologous nasopharyngeal carcinoma cells CNE - 2R and CNE - 2 with different radioresistance.METHODS : The effects of B50 on cell viability and cell growth were detected by MTT assay and colony - forming experiment, respectively.The changes of cell cycle, apoptosis and mitochondrial membrane potential ( MMP ) were determined by flow cytometry.RESULTS : B50 inhibited the cell viability of CNE - 2R cells in a time - and dose - dependent manner with the IC50 of ( 8.06±0.14 ) μmol/L ( 24 h ), ( 2.49±0.02 )μmol/L ( 48 h ) and ( 1.42 ±0.02 ) μmol/L ( 72 h ), which was more effective than that in CNE -2 cells [with the IC50 of ( 8.18 ±0.12 ) μmol/L ( 24 h ), ( 3.00 ±0.04 ) μmol/L ( 48 h ) and ( 1.75 ±0.01 ) μmol/L ( 72 h )].The inhibitory effect of B50 on CNE -2R cell growth was more effective than that on CNE -2 cells [the IC50 of 48 h was ( 0.55 ±0.01 ) μmol/L vs ( 0.82 ±0.01 ) μmol/L].After treated with B50 for 48 h, the proportion of CNE - 2R cells in G2/M stage was increased from 7.1％ to 34.9％ , which was better than that of CNE -2 cells ( from 12.4％ to 35.7％ ).After treated with B50 for 24 h, the early apoptotic rate in CNE -2R cells was increased from 3.7％ to 19.5％ , which was better than that in CNE -2 cells ( from 4.4％ to 14.8％ ), and the MMP in CNE -2R cells was decreased by ( 43.17 ± 3.11 )％ , which was better than that in CNE - 2 cells[( 35.06 ±1.07 )％].CONCLUSION : B50 is more effective on inhibiting the cell viability and cell growth, blocking the cell cycle at G2/M stage, inducing apoptosis and decreasing MMP in CNE - 2R cells than those in CNE - 2 cells, indicating that B50 may enhance the radio - sensitivity of CNE - 2R cells by blocking the cell cycle and inducing apoptosis through mitochondrial pathway.%目的:比较姜黄素衍生物(B50)抑制不同辐射抗拒的同源鼻咽癌细胞CNE-2与CNE-2R作用的差异.方法:采用MTT法和克隆形成实验检测B50对细胞活力及增殖的影响,以流式细胞仪检测B50对细胞周期分布、细胞凋亡及线粒体膜电位的影响.结果:B50可抑制CNE-2R细胞的活力[24 h、48 h、72 h的IC50分别为(8.06±0.14)、(2.49±0.02)、(1.42±0.02)μmol/L],且呈时间浓度依赖性,其抑制作用明显优于对CNE-2细胞的作用[24 h、48 h、72 h的IC50分别为(8.18±0.12)μmol/L、(3.00±0.04)μmol/L、(1.75±0.01)μmol/L],P<0.01;同时B50抑制CNE-2R细胞增殖的作用明显优于对CNE-2的作用[48 h的IC50分别为(0.55±0.01)μmol/L vs (0.82±0.01)μmol/L],P<0.01;药物作用48 h后,B50使CNE-2R细胞G2/M期的比例升高(7.1%→34.9%),明显优于CNE-2细胞 (12.4%→35.7%)(P<0.01);B50作用于CNE-2R细胞24 h后早期凋亡率升高(3.7%→19.5%),明显优于CNE-2细胞(4.4%→14.8%)(P<0.01);B50作用24 h后,CNE-2R细胞线粒体膜电位下降了(43.17±3.11)%,明显优于对CNE-2细胞的降低率[(35.06±1.07)%](P<0.01).结论:与CNE-2相比,B50能更显著地抑制辐射抗拒CNE-2R细胞的细胞活力及增殖能力,且更明显地调控细胞周期停滞于G2/M期,诱导细胞凋亡及降低线粒体膜电位.这些结果提示B50具有逆转辐射抗拒的潜在价值.
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