不同人结直肠肿瘤细胞系对常用抗肿瘤药物体外化疗敏感性的比较

摘要

AIM; To observe the antitumor effect of 5 commonly used chemotherapeutic drugs on 11 human colorectal cancer cell lines in vitro. METHODS: CCK - 8 method was used to determine the growth inhibitory effects of 5 antitumor drugs, which were expressed as the half growth inhibitory concentration (IC50 ) and sensitivity index IC50/PPC ( peak plasma concentration ) on 11 human colorectal cancer cell lines . The expression variations of heat - shock protein 27 ( HSP27) and HSP70 at protein levels in human colorectal tumor cell lines treated with different chemotherapeutic drugs were observed by Western blotting. RESULTS; All the 11 colorectal cancer cell lines were sensitive to 5 - fluorouracil (5 -FU) and oxaliplatin (OHP) without drug resistant. Five colorectal cancer cell lines were sensitive to mitomycin ( MMC) , while the other 6 cell lines were moderately sensitive. Ten colorectal cancer cell lines except SW 1116 were sensi-rntive to docetaxel (DXL) , while SW1116 cells were resistant to DXL. Nine colorectal cancer cell lines except LS 174T and SW1116 were moderately sensitive to irinotecan (IFL) , and SW1116 cells were also resistant to IFL, while LS174T cells were sensitive to IFL. After treated with the antitumor drugs , HSP27 was up - regulated in HCT116 cells and SW480 cells, while the expression of HSP70 didn't change. CONCLUSION; LS174T cells are multidrug - sensitive, while SW1116 cells are multidrug -resistant. 5 - FU and OHP are the wide - spectrum anti - colorectal cancer drugs. Determining the sensitivity to chemotherapeutic drugs and the expression level of HSP 27 can improve the accuracy in drug selection .%目的:观察5种常用抗肿瘤药物对这些人结直肠肿瘤细胞系的生长抑制作用,探讨5种常用抗肿瘤药物对11株人结直肠肿瘤细胞系的作用强度以及比较其体外敏感性,研究不同抗肿瘤药物对人结直肠癌细胞系HCT116和SW480热休克蛋白27(HSP27)和HSP70表达水平的影响.方法:采用CCK-8(Cell Counting Kit-8)法检测5种常用抗肿瘤药物分别对11株人结直肠肿瘤细胞系的生长抑制效应,计算50%抑制浓度(50% inhibitory concentration,IC50)及敏感指数,并比较不同人结直肠肿瘤细胞系对5种抗肿瘤药物的敏感性,Western blotting检测HSP27和HSP70蛋白表达水平.结果:11株人结直肠肿瘤细胞系对5-氟尿嘧啶(5-FU)和奥沙利铂(OHP)均比较敏感,没有明显耐药性;5株人结直肠肿瘤细胞系对丝裂霉素(MMC)敏感,6株中度敏感;除SW1116 外的10株人结直肠肿瘤细胞系都对多西紫杉醇(DXL)敏感,而SW1116细胞对DXL表现出明显耐药性;除LS174T和SW1116外的9株人结直肠肿瘤细胞系都对伊立替康(IFL)表现出中度敏感,LS174T细胞对IFL表现敏感,而SW1116细胞对IFL表现出明显耐药性.抗肿瘤药物作用于人结直肠癌细胞系HCT116和SW480使HSP27的表达上调,但HSP70的表达水平变化不明显.结论:LS174T是多药敏感细胞株,SW1116是多药耐药细胞株,5-FU和OHP为广谱抗结直肠肿瘤药物;化疗药物的敏感性及HSP27表达量检测对临床选择化疗药物具有一定的提示意义.