扶肾降浊方含药血清对肾小管间质损害大鼠成纤维细胞抗纤维化因子 HGF 和 BMP-7的影响

摘要

AIM:To study the protective effects and mechanism of Fushen Jiangzhuo formula (FSJZ) on the rat renal interstitial fibroblasts with renal tubulointerstitial lesion .METHODS:The serum containing FSJZ and blank con-trol serum were prepared .The rat model of mesangial proliferative glomerulonephritis ( MsPGN) was established and ex-tended the time of modeling to 20th weeks for developing tubulointerstitial lesion naturally .The rat renal interstitial fibro-blasts at the end of 12, 16, 20 week of modeling were isolated and cultured .The effects of FSJZ on the expression of hepa-tocyte growth factor (HGF) and bone morphogenetic protein-7 (BMP-7) at mRNA and protein levels in the pathological re-nal interstitial fibroblasts were determined .RESULTS:The anti-fibrosis factors HGF and BMP-7 were changed in patho-logical renal interstitial fibroblasts .Renal tubulointerstitial lesion significantly down-regulated the expression of HGF and BMP-7, while the drug containing serum of FSJZ significantly up-regulated the expression of HGF and BMP-7.CONCLU-SION:Drug containing serum of FSJZ has protective effect on pathological renal interstitial fibroblasts by regulating the mRNA and protein expression of HGF and BMP-7.%目的：探讨扶肾降浊方对系膜增生性肾小球肾炎（ MsPGN）大鼠肾小管间质损害的疗效及机制。方法：采用扶肾降浊方水溶液灌胃Wistar大鼠常规制备含药血清，在MsPGN动物模型的基础上，延长造模时间至20周，使其自然发展为肾小管间质损害模型，体外培养造模12、16和20周末大鼠间质成纤维细胞，采用real-time PCR和Western blotting法检测扶肾降浊方含药血清对病理状态间质成纤维细胞中抗纤维化因子肝细胞生长因子（ HGF）、骨形态发生蛋白-7（ BMP-7） mRNA及蛋白表达的影响。结果：病理状态间质成纤维细胞中抗纤维化因子HGF、BMP-7 mRNA和蛋白表达下调，扶肾降浊方含药血清随着给药周期的延长可部分逆转间质损害造成的上述mRNA和蛋白表达异常。结论：扶肾降浊方含药血清对MsPGN大鼠间质成纤维细胞的保护作用可能与调节抗纤维化因子HGF、BMP-7 mRNA和蛋白表达有关。