不同光照对雌性抑郁大鼠生殖系统的影响

摘要

AIM:To investigate the effects of different lighting on the reproductive system in depressive female rats.METHODS:Healthy adult female rats were randomly chosen as control group , and the depressive adult female rats in SPF grade were randomly divided into 5 groups (7 rats each):depressive model group, sulfur lamp group, heat radia-tion lamp group , fluorescent lamp group and LED lamp group .After 45 d of continuous illumination , the estrous cycle was observed by the vaginal exfoliated cells , and the organ indexes of ovary and uterus were calculated .The concentrations of estiadrol (E2), prolactin (PRL), progesterone (PROG) and testosterone (T) in the serum were detected by ELISA, and the histopathological lesion of ovary was observed under microscope with HE staining .RESULTS:The estrous cycle exhib-ited serious disorder , the ovaries exhibited serious congestion , and the organ indexes of ovary and uterus and the concentra-tions of E2 , PRL, PROG and T decreased significantly in the rats in depressive model group compared with control group (P<0.05).The estrous cycle and histopathological damage of ovary were obviously improved , and the concentrations of E2 , PRL, PROG and T were significantly increased after the sulfur lamp lighting in the depressive female rats compared with depressive model group .No obvious change and improvement of the reproductive functions in the heat radiation lamp group, fluorescent lamp group and LED lamp group was observed .CONCLUSION:The reproductive functions of depres-sive female rats are improved by sulfur lamp lighting .%目的：研究不同光照对雌性抑郁大鼠生殖系统的影响。方法：将35只已成功建立抑郁模型的成年SPF级SD雌性大鼠随机分为抑郁模型组、微波硫灯组、热辐射灯组、日光灯组和LED灯组，并设立健康成年SPF级SD雌性大鼠为对照组，每组7只。在连续光照45 d后，阴道涂片法检测生殖周期，计算子宫和卵巢脏器系数比， ELISA方法检测血清中雌二醇（E2）、催乳素（PRL）、孕酮（PROG）及雄激素睾酮（T）含量，HE染色观察卵巢的组织病理学。结果：与对照组相比，抑郁模型组大鼠生殖周期严重紊乱，子宫和卵巢脏器系数比明显下降（P＜0.05）， E2、PRL、PROG及T的含量降低（P＜0.05），卵巢充血严重。微波硫灯照射可以改善雌性抑郁大鼠的生殖周期，上调E2、PRL、PROG及T水平，减轻卵巢充血组织病理学改变，热辐射灯、日光灯和LED灯照射对雌性抑郁大鼠的生殖周期、性激素水平及卵巢组织病理学变化没有明显作用。结论：微波硫灯照射可以改善雌性抑郁大鼠的生殖功能。