低表达miR-21对苦参碱诱导的肝癌HepG2细胞凋亡的影响

摘要

目的：探讨miR-21低表达能否增强苦参碱（ matrine，MAT）对肝癌细胞的促凋亡作用。方法：实时荧光定量PCR（RT-qPCR）检测不同浓度MAT作用HepG2细胞后miR-21的表达情况。流式细胞术检测miR-21低表达对MAT诱导的细胞凋亡的影响；RT-qPCR和Western blot检测miR-21低表达对MAT诱导的细胞凋亡中Bcl-2和Bax mRNA和蛋白表达水平的影响。结果：miR-21表达量随MAT作用浓度的增加而增加；miR-21低表达可促进MAT诱导的细胞凋亡，并促进Bax mRNA和蛋白的表达（ P＜0.05），而抑制Bcl-2 mRNA和蛋白的表达（ P＜0.05）。结论：miR-21低表达可通过抑制Bcl-2和促进Bax的表达来增强MAT诱导的HepG2细胞凋亡。%AIM:To explore whether miR-21 low expression enhances the effect of matrine ( MAT) on the ap-optosis of hepatocellular carcinoma cells .METHODS:Real-time fluorescence quantitative PCR ( RT-qPCR) was used to detect the expression of miR-21 in the HepG2 cells treated with different concentrations of MAT .The effect of miR-21 on MAT-induced HepG2 cell apoptosis was analyzed by flow cytometry .The mRNA and protein expression of Bcl-2 and Bax in the HepG2 cells treated with MAT was determined by RT-qPCR and Western blot .RESULTS:The expression of miR-21 increased with the increasing concentration of MAT .Low expression of miR-21 promoted MAT-induced apoptosis , and en-hanced the expression of Bax at mRNA and protein levels ( P<0.05 ) , while inhibited the expression of Bcl-2 at mRNA and protein levels (P<0.05).CONCLUSION:Low expression of miR-21 enhances MAT-induced HepG2 cell apoptosis by inhibiting the expression of Bcl-2 and promoting Bax expression .