Method for preparing purified extract of Citrus spp. fruit peels having an effect of inducing apoptosis of hepatocellular carcinoma cell

摘要

The present invention relates to a method for producing citrus dermabrasion extracted tablets having the effect of inducing apoptosis of liver cancer cells. More specifically, the present invention relates to a method for producing citrus dermabase extracted tablets by using 50-90% (v / v) methanol extraction, The present invention relates to a method for producing a citrus fruit extract extract which inhibits cell viability of liver cancer cells, affects the cell cycle, and induces apoptosis of cells.In accordance with the present invention, there is provided a method of inhibiting the expression of Bak protein, suppressing expression of Bcl-xL protein, inhibiting Caspase family protein expression, inhibiting expression of PARP protein, inhibiting Akt protein phosphorylation, increasing phosphorylation of P38 MAPK protein and phosphorylation of JNK protein Or by inducing apoptosis of the cells through increased phosphorylation of the ERK protein, thereby producing citrus dermabase-purified tablets which can effectively prevent, ameliorate or treat liver cancer. The manufacturing method of the present invention also has an effect that a usable material can be manufactured at a very low cost because the raw material to be discarded is used. In addition, it is expected that the use of citrus dermis can be economically beneficial for citrus farmers.;