鼻咽上皮癌变过程中端粒酶活性和端粒酶RNA的表达

摘要

目的研究二亚硝基哌嗪（DNP）诱发大鼠鼻咽癌变过程中端粒酶的表达规律。方法以DNP诱导大鼠鼻咽癌；用PCR-ELISA 和Nested RT-PCR检测DNP诱导大鼠鼻咽癌变不同阶段端粒酶活性和端粒酶RNA的表达，同时作病理形态学检测。结果 DNP诱导大鼠鼻咽癌变过程中，端粒酶活性不断升高，端粒酶的变化与鼻咽癌变呈正相关，而且端粒酶中RNA表达先于端粒酶的表达。在大鼠鼻咽上皮细胞异型增生阶段即出现端粒酶的激活和端粒酶RNA的表达。结论化学致癌物DNP诱导细胞癌变端粒酶的激活，且端粒酶的激活和端粒酶RNA的表达是鼻咽上皮癌变的早发事件，与鼻咽癌的发生、发展有关。%Objective　 To investigate the profile of telomerase and telomerase RNA expression in nasopharyngeal carcinogenesis (NPC) induced by N,N'dinitrosopi perazine (DNP) and examined histolofically. MethodsrnNasopharyngeal carcinomas o f rats were induced by DNP and examined histologically. PCR-ELISA and nested R T -PCR were used to assay telomerase and telomerase RNA expression at different s tages in the nasopharyngeal tissues of rats. Results　 During the carcinogenesis p rocess, telomerase activity increased along with the formation of a nasopharynge al carcinoma. Telomerase expression was positively related with nasopharyngeal c arcinogenesis. Telomerase RNA expression was present and did not change during t he NPC process. Expression of telomease RNA was earlier than telomerase activat ion. Telomerase activation and telomerase RNA expressin were also detected in th e pre-cancerous nasopharyngeal lesions. Conclusion　 Telomerase activation may p articipate in the onset and progression of NPC, and is an early step in NPC.