丙泊酚麻醉下大鼠反复腰椎穿刺鞘内给药的可行性研究

摘要

目的:探讨丙泊酚麻醉下大鼠腰椎水平反复穿刺鞘内给药的可行性,为大鼠鞘内多次给药提供一种新方法.方法:成年雄性SD大鼠(250～300g)24只,随机分为正常组、丙泊酚组、腰椎穿刺组和腰椎穿刺并给生理盐水组,每组6只.采用丙泊酚(50mg/kg)麻醉大鼠后,用50μl微量进样器于腰5～6间隙行经皮腰椎穿刺,以大鼠后肢出现颤动或鼠尾侧向摆动为穿刺成功的标志.实验中测定大鼠热甩尾潜伏期、免疫荧光法检测脊髓星形胶质细胞和小胶质细胞变化.结果:丙泊酚组、腰椎穿刺组及腰椎穿刺并给生理盐水组大鼠热甩尾潜伏期与对照组相比差异均无统计学意义(P>0.05),且其脊髓的星形胶质细胞和小胶质细胞活化情况较正常对照组亦无统计学改变(P>0.05).结论:丙泊酚麻醉下大鼠反复腰椎穿刺鞘内给药法安全有效,可用于需多次鞘内给药,并在给药后立刻检测大鼠疼痛行为学指标的实验.%Objective:The feasibility of repeated intrathecal administration via lumbar puncture with propofol anesthesia was investigated to provide a new method for repeated intrathecal administration in rats. Methods; 24 Sprague-Dawley rats (male, 250～300g) were randomly divided into naive group, propofol group, lumbar puncture group and lumbar puncture with saline injection group, 6 rats in each group. Rats were anesthetized with propofol (50 mg/kg), then injected transcutaneously at the L5-6 interspace using a 50 μl Hamilton syringe. Correct subarachnoid positioning of the tip of the needle was verified by a tail- or paw-flick test. In the experiment, the thermal tail-flick latencies, changes of astrocytes and microglia cells in the spinal cord were determined. Results:No significant difference of thermal tail-flick latencies was found between the propofol group, lumbar puncture group, lumbar puncture with saline injection group compared to the naive group (P >0.05). There was no significant difference of astrocytes and microglia cells in the spinal cord between experiment rats and the control rats (P>0.05) . Conclusion; Repeated intrathecal administration via lumbar puncture in rats with propofol anesthesia is safe and effective. This method can be used in the experiments that need repeated intrathecal administration and immediate testing of pain behavior in rats after intrathecal administration.