p16、COX-2在喉癌组织中的表达及相关性研究

摘要

目的 研究喉癌组织中抑癌基因p16及环氧化酶-2(COX-2)的表达,探讨p16、COX-2在喉癌发生发展中的作用及两者之间的关系.方法 采用免疫组织化学方法检测50例喉癌组织中p16、Cox-2的表达情况.结果 50例喉癌标本中喉癌中p16蛋白表达的阳性率为56.0%,正常喉黏膜中阳性表达率为80.0%,p16蛋白在喉鳞癌组织中的阳性表达率明显低于正常喉黏膜组织(P<0.05),有统计学意义.随着临床分期的进展及组织学分化程度的降低,p16蛋白表达进一步降低.COX-2在喉癌组织中高表达,阳性表达率为62.0%,正常组织阳性率为24%,患者的不同临床分期的表达差异有统计学意义(P<0.05).p16与COX-2之间存在负相关性.结论 两者的联合检测对估计喉癌的恶性度,判断预后有FINDINGS REPORT定意义.%Objective To investigate the expression of p16 and cyclooxygenase-2 (Cox-2) in laryngeal squamous carcinoma and analyze the relationship p16 and Cox-2. Methods 50 cases of laryngeal squamous carcinoma were detected by using immunohistochemistry. Results The positive rate of pi6 expression in laryngeal squamous carcinoma was 56.0％, but it was 80.0％ in the normal laryngeal mucosa. The positive rate ofpl6 in laryngeal squamous carcinoma were significantly lower than that in the normal tissues (P＜ 0. 05). Furthermore, pi6 expression showed a declined tendency in the positive rate in cases with the aggravation of clinical stag and cell differentiation. Ovaexpression of Cox-2 was found in the laryngeal squamous carcinoma specimens. The positive rate of Cox-2 was 62.0％, and it was related to the clinical stage and the histological grade, had a negative correlation with p16. Conclusions Combinative detection of p16 and cyclooxygenase-2 could have significance in predicting the biological aggressiveness and clinical outcome of laryngeal carcinoma.