COX-2及P16在非霍奇金淋巴瘤中的表达及临床意义

摘要

Objective:To investigate the expression of cyclooxygenase-2(COX-2)and p16 proteins in non-Hodgkin's lym phomas(NHL)and their relationship with the genesis and progress of it.Methods:The expression of COX-2 and p16 protein were studied in the lymph nodes tissue from 60 NHL patients and 10 control patients with non-malignant diseases by flow cytometry.Results:Positive rate of COX-2 protein expression in NHL tissues(63.3%.38/60)was higher than that in normal lymphaden tissues(0,0/10).The difiefence was significant between the two groups(P＜0.01).Expression of COX-2 protein was related with the clinical stage of NHL.In stage Ⅰ+Ⅱ patients,it was significantly lower(35.0%+54.6%)than that in stage Ⅲ+Ⅳ patients(84.6%+87.5%)(P＜0.01).In different sex,age,tumor malignant degree,IPI grade,extranodal involvement and B symptoms groups,the differences of COX-2 expression were not statistically significant (P＞0.05).Positive fate of p16 protein expression(41.7%,25/60)in NHL' was statistically lower than that in normal lymphomas(100%,10/10)(P＜0.01). Expression of p16 protein was related to malignant degree of NHL.The positive rates of p16 protein in low malignant degree tissues(64.7%,11/17)was higher than that in high malignant degree tissues(14.3%,2/14)(P＜0.05).Positive rates of p16 protein of NHL tissues in different sex.age.IPI grade,extranodal involvement,dinical stages and B symptoms were not statistically significant(P＞0.05).The p16 protein expression in COX-2 positive patients was 47.4%(18/38),and in negative patients it was 31.8%(7/22).There was no statistically difference between them(P＞0.05).Correlation analysis revealed there was no correlation between expression of COX-2 and p16 protein.Conclusion:B0th COX-2 and P16 protein may all have relationship with the genesis and progress of NHL.The expression of COX-2 protein in NHL may be a poor prognostic indicator.COX-2 and p16 protein probably have dilferent mechanisms in the genesis and progress of NHL.Their relationship is firstly put forward in this article and needed further studying.