β防御素在中医烙法治疗慢性扁桃体炎组织中的表达及临床意义

摘要

Objective To detect expression of human β-defensin-1(hBD-1) and humanβ-defensin-2(hBD-2) in the normal,erythromycin,pro-treatment,cauterization treatment,post-treatment of cauterization,and to evaluate the significance of β-defensin in cauterization treatment on chronic tonsillitis.Method To detected mRNA expression of hBD-1 and hBD-2 in the tonsil of each group with real-time quantitative PCR.Result The hBD-1mRNA was expressed in all tissue specimens,there was on significant difference (P＞0.05) at level.hBD-2 mRNA in tonsil tissue of the post-treatment group was significantly increased compared to the other groups (P＜0.05).The other groups showed no significant difference (P＞0.05).Conclusion The hBD 1 was innate expression,and it may can play a natural defensive function.Cauterization can induce hBD-2 expression in the tonsil tissue,so as to achieve treatment effect.%目的 检测β防御素hBD-1及hBD-2mRNA在正常组、红霉素组、烙前组、烙后即刻组、烙后组扁桃体组织中的表达,探讨β防御素在中医烙法治疗慢性扁桃体炎中的意义.方法 应用实时荧光定量PCR技术检测各组扁桃体中hBD-1及hBD-2mRNA的表达.结果 hBD-1 mRNA在各组中均有表达,且各组间差异无统计学意义(P＞0.05)；hBD-2mRNA在烙后组扁桃体组织中表达较其它各组表达明显增强(p＜0.05),其它各组比较无显著性差异(P＞0.05).结论 hBD-1呈固有性表达,能发挥天然防御作用；hBD-2呈诱导性表达,中医烙法可提高扁桃体组织中的hBD-2的表达水平,从而达到治疗的目的.