大鼠耳蜗缺血再灌注后APE/Ref-1表达研究

摘要

Objective To investigate the expression of APE/Ref-1 in cochlear following cochlear ischemia reperfusion injury in rats. Methods Twenty four healthy SD rats were randomly divided into 2 groups, namely, the sham operation control group and the ischemia reperfusion group .There were 12 rats in each group. The expression of APE/Ref-1 in cochlea was measured immunohistochemistry and western blot . Results In the ischemia reperfusion group, the content of APE/Ref-1 was increased significantly compared with those in the control group. In the normal SD rats, APE/Ref-1 was highly expressed in the cytoplasm and nucleus of spiral ganglion cells and also distributed in the area of spiral ligament and stria vascularis. In the ischemia reperfusion group, The expression of APE/Ref-1 in cochlea was increased significantly in cochlear. Coclusion APE/Ref-1 was highly expressed in the spiral ganglion cells. And it was also observed the content of APE/Ref-1 was in-creased significantly In the ischemia reperfusion group. These results suggests that APE/Ref-1 is closely involved in the oxi-dative stress of ischemia reperfusion injury in the cochlear.%目的：观察APE/Ref-1在正常成年SD大鼠内耳蜗缺血再灌注后的表达变化。方法成年SD大鼠24只，随机分成2组（n＝12），分别为假手术对照组、缺血再灌注组。各组动物于再灌注24h取出左侧耳蜗，每组随机4个耳蜗，石蜡包埋切片，行免疫组织化学观察APE/Ref-1表达分布。各组余下耳蜗组织进行均浆，进行免疫印记实验，测定匀浆中APE/Ref-1蛋白表达。结果与假手术对照组相比，缺血再灌注组APE/Ref-1蛋白表达显著增加（P＜0.05）。在假手术组大鼠耳蜗中，APE/Ref-1主要表达在正常螺旋神经节区，细胞内定位在细胞浆和细胞核，螺旋韧带和血管纹也可以见到表达。缺血再灌注组APE/Ref-1在耳蜗这些部位的表达显著增加。结论正常大鼠内耳表达APE/Ref-1，主要表达在螺旋神经节区。耳蜗缺血再灌注后，APE/Ref-1在内耳的表达显著增加，提示APE/Ref-1可能与耳蜗缺血再灌注氧化损伤密切相关。