氟砷联合作用对大鼠骨密度影响的实验研究

摘要

Objective To explore the combined effects of fluoride and arsenic on bone mineral density (BMD) in chronic fluoride-arsenic poisoned rats.Methods Factorial design was applied in the experiment.One hundred and sixty 6-week-old Wistar rats were randomly divided into 16 groups: low-,middle-, or high-dose of fluoride group; low-, middle-, or high-dose of arsenic groups; low-dose of fluoride and arsenic group; middle-dose of fluoride and arsenic group; high-dose of fluoride and arsenic group; low-dose of fluoride and middle-dose of arsenic group; low-dose of fluoride and high-dose of arsenic group;middle dose of fluoride and high-dose of arsenic group; high-dose of fluoride and low-dose of arsenic group;high-dose of fluoride and middle-dose of arsenic group; and control group.Ten rats, half of males and half of females, were in each group.Water containing high-dose (200mg/L), middle-dose (l00mg/L) , or low-dose of fluoride (50mg/L) , high-dose (34mg/L) , middle-dose (8.5mg/L) , or low-dose (2.125mg/L) of arsenic was freely accessed by rats in the corresponding group for 6 months.Rats in the control group drank distilled water freely.BMDs of both femurs were examined with dual energy X-ray densitometry after 2-, 4-,or 6-month of feeding.Results The effect of fluoride on BMD was significant (P ＜ 0.05).The effect of arsenic on BMD was not significant (P ＞ 0.05).The combined effects of fluoride-arsenic on BMD were not insignificant ( P ＞ 0.05).Conclusion No obvious combined effects of arsenic and fluoride were shown on BMD.The alteration of BMD by fluoride was due to its effect on bone resorption, formation, and mineral deposition.Arsenic can not affect BMD, and the impact of fluoride on BMD, either.%目的 探讨在慢性氟砷染毒实验中观察氟砷联合作用对大鼠骨密度的影响.方法 实验采用析因设计,将160只6周大的Wistar大鼠随机均分至16组:低氟、中氟、高氟组,低砷、中砷、高砷组,低氟低砷、中氟中砷、高氟高砷组,低氟中砷、低氟高砷、中氟高砷,中氟高砷、高氟低砷、高氟中砷组及对照组.每组10只,雌雄各半,自由饮用含氟(高氟200 mg/L、中氟100 mg/L、低氟50mg/L)、砷(高砷34 mg/L、中砷8.5 mg/L、低砷2.125mg/L)及其相应混合的水溶液.对照组饮用蒸馏水.喂养2个月、4个月和6个月后使用双能X线骨密度仪测量大鼠双侧股骨骨密度.结果 氟对骨密度的影响明显(P＜0.05);砷对骨密度影响不显著(P＞0.05);氟砷交互作用(P＞0.05)不显著.结论 砷与氟在对骨密度改变上无明显联合作用.对骨密度的改变是氟对骨吸收、骨形成、骨矿化都起促进作用,而砷不影响骨密度,亦不影响氟致骨密度改变.