中医不同治法对骨质疏松症大鼠骨密度及骨骼肌Ca2+-Mg2+-ATP酶影响的比较研究

摘要

Objective To observe the effect of the different treating methods with traditional Chinese medicine (TCM) on bone mineral density (BMD) and Ca2+ -Mg2+-ATP enzyme in the skeletal muscle in rats with glucocorticoid-induced osteoporosis and to discuss the mechanism of TCM on prevention and treatment of osteoporosis.Methods One hundred and twenty rats with half of each sex were randomly divided into 6 groups, including normal control group, model control group, reinforcing the kidney herb group, strengthening the spleen herb group, promoting blood circulation to remove stasis herb group, and GUSUKANG herb group.Intramuscular injection of dexamethasone was use to establish the model.At the end of the experiment, the rats were killed by withdrawing blood from the abdominal aorta.Ca2+ -Mg2+ -ATP enzyme was measured using enzyme-linked immunosorbent assay and BMD of the upper 1/3 of rat femur was detected using dual energy X-ray absorptiometry.Results ①Compared to normal control group, BMD of upper 1/3 of the femur in model control group decreased significantly (P ＜ 0.01 ).Compared to model control group, BMD of upper 1/3 of the femur increased in all treatment groups at different degrade, with the most significant increase in reinforcing the kidney herb group ( P ＜ 0.01 ).The BMD difference between model control group and the other treatment groups had no statistical significance.②Compared to normalcontrol group, Ca2+ -Mg2+ -ATP enzyme in the skeletal muscle in all other groups decreased significantly (P ＜ 0.01 ).Compared to model control group, Ca2+-Mg2+ -ATP enzyme in the skeletal muscle of all treatment groups increased significantly ( P ＜ 0.01 ).Ca2+ -Mg2+ -ATP enzyme in the skeletal muscle in reinforcing the kidney herb group increased the most significantly, compared to GUSUKANG group, promoting blood circulation group, and strengthening the spleen group with statistical significance (P ＜ 0.01 ).The increase of Ca2+-Mg2+-ATP enzyme in the skeletal muscle in promoting blood circulation group was the lowest,compared to that in reinforcing the kidney herb group, strengthening the spleen group, and GUSUKANG group (P ＜ 0.01 ).Those increases in strengthening the spleen group and GUSUKANG group were also apparent.However the difference between the two groups was no statistical significance.Conclusion The reinforcing the kidney method and the strengthening the spleen method had certain regulative effect on BMD and Ca2+-Mg2+ -ATP enzyme in the skeletal muscle in osteoporotic rats.%目的 观察中医不同治法对糖皮质激素诱导骨质疏松症大鼠骨密度、骨骼肌Ca2+-Mg2+-ATP酶变化的影响,探讨中医防治骨质疏松症的作用机制.方法 将120只雌雄各半的大鼠随机分为正常对照组、模型对照组(模空组)、补肾中药组、健脾中药组、活血化瘀中药组和骨疏康中药组6个组.用地塞米松肌注造模.实验结束后,腹主动脉取血处死大鼠,用酶联免疫法测定大鼠骨骼肌Ca2+-Mg2+-ATP酶,用双能X线骨密度仪测大鼠离体股骨上1/3骨密度.结果 ①与正常组比较,模空组大鼠离体股骨上1/3骨密度显著降低(P<0.01);与模空组比较,各治疗组大鼠股骨上1/3骨密度均有不同程度的升高,其中以补肾中药组升高程度最为显著(P<0.01),其余各治疗组骨密度较模空组升高程度比较无统计学意义.②与正常组比较,其他各组大鼠骨骼肌的Ca2+-Mg2+-ATP酶显著降低(P<0.01);与模空组比较,各治疗组大鼠骨骼肌的Ca2+-Mg2+-ATP酶均明显升高(P<0.01);补肾组大鼠骨骼肌的Ca2+-Mg2+-Mg2+-ATP酶升高最为明显,明显高于骨疏康组、活血组、健脾组,差异具有非常显著性(P<0.01);活血组大鼠骨骼肌的Ca2+-Mg2+-ATP酶升高程度最低,与补肾组、健脾组、骨疏康组比较具有统计学差异(P<0.01);健脾组和骨疏康组升高程度也比较明显,但二者比较无统计学意义.结论 补肾、健脾方法对骨质疏松症大鼠的骨密度和Ca2+-Mg2+-ATP酶具有一定调节作用.