中医不同治法对绝经后骨质疏松症大鼠骨骼、骨骼肌甲状旁腺激素相关蛋白(PTHrP)含量影响的比较研究

摘要

目的 观察补肾、健脾、活血法对骨质疏松症大鼠骨骼、骨骼肌甲状旁腺激素相关蛋白(PTHrP)含量变化的影响.方法 将SD雌性大鼠分为正常组、模型组、补肾组、健脾组、活血组.采用酶联免疫法测定小鼠骨骼、骨骼肌PTHrP含量变化.结果 (1)与正常组比较,模型组大鼠骨骼的PTHrP含量显著降低(P＜0.01);与模型组比较,补肾组、健脾组、活血组骨骼的PTHrP含量明显升高(P＜0.01),其中补肾组升高程度比较明显.(2)与正常组比较,模型组大鼠骨骼肌的PTHrP含量显著降低(P＜0.01);与模型组比较,补肾组、健脾组、活血组骨骼肌的PTHrP含量明显升高(P＜0.01),其中健脾组升高程度比较明显.结论 (1)骨质疏松症的形成,可能与骨骼、骨骼肌协调性下降有关.(2)补肾、健脾方法通过提高骨质疏松症大鼠的骨骼、骨骼肌PTHrP含量,对骨质疏松症具有一定的防治作用.%Objective To evaluate the effects of CTM regimen including tonifying the kidney, invigorating the spleen and promoting blood circulation on the protein expression of of PTHrP in bone and skeletal muscle tissues of rats with osteoporosis.Methods Rats were randomly divided into normal control group, model control group, tonifying kidney group, invigorating the spleen group and promoting blood circulation group.All the indexes were determined by ELISA method.Results (1) Compared with the normal group, the content of PTHrP enzyme in bone tissues of the model control group decreased (P ＜ 0.01);compared with the model control group, the content of PTHrP enzyme in bone tissues of the tonifying kidney group, invigorating the spleen group and promoting blood circulation group increased.(2) Compared with the normal control group, the content of PTHrP enzyme in skeletal muscle tissues of the model control group decreased (P ＜ 0.01);compared with the model control group, the content of PTHrP enzyme in skeletal muscle tissues of tonifying kidney group, invigorating the spleen group and promoting blood circulation group increased.Conclusion (1) Osteoporosis maybe related to the harmony of bone and skeletal muscle.(2) The methods of tonifying kidney and invigorating the spleen have certain preventive effects on osteoporosis by regulating the protein expression of PTHrP in bone and skeletal muscle tissues.