MMP-3、MMP-13在骨性关节炎患者滑膜中的表达及意义

摘要

Objective To investigate the expression of MMP-3/-13 in the synovium in patients with osteoarthritis and their correlation.Methods Thirty patients with osteoarthritis ( OA, experiment group) and 30 patients without OA ( normal group) were selected.The expression of MMP-3/-13 in the synovium in both groups was detected using immunohistochemistry method and Western blotting method.Results The results of immunohistochemistry method showed that, the expression of MMP-3/-13 in experiment group was significantly higher than that in normal group (P<0.01).And the positive expression of MMP-3/-13 mainly located in the cytoplasm.The results of Western blotting showed that the expression of MMP-3/-13 in experiment group was significantly higher than that in normal group.And the expression of MMP-3 was positively correlated with the expression of MMP-13 in experiment group.Conclusion The expression of MMP-3/-13 is closely correlated with the development of OA, which may be an important indicator in the diagnosis and judgment of OA.%目的：探讨MMP-3、MMP-13在骨性关节炎患者滑膜中的表达及相关性。方法应用免疫组化和Western blotting法检测MMP-3、MMP-13蛋白在30例骨性关节炎（ OA，实验组）与30例非OA（对照组）患者滑膜组织中的表达情况。结果免疫组化结果表明MMP-3、MMP-13蛋白在实验组与对照组患者滑膜中的表达有显著性差异（ P＜0.01），且阳性表达多定位于滑膜细胞的胞浆；Western blotting分析结果显示MMP-3、MMP-13蛋白在实验组患者滑膜中表达明显高于对照组患者，且二者在OA组中表达呈正相关性。结论 MMP-3、MMP-13蛋白与骨性关节炎发生发展密切相关，可能作为诊断及判断骨性关节炎的一项重要指标。