Objective To analysis the levels of interleukin ( IL )-17 and matrix metalloprotienase ( MMP )-3 in synovial fluid and plasma in patients with rheumatoid arthritis ( RA ) and to investigate it' s clinical significance. Methods The levels of IL-17 and MMP -3 in plasma and synovial fluid in RA patients were tested by ELISA, simultaneously, The relationships among IL-17, MMP-3 and other clinical indexes were analyzed. Results IL-17 and MMP-3 levels in synovial fluid in patients with RA were significantly higher than those in control groups ( P ＜ 0.01 ), the levels of plasma IL-17 and MMP-3 in active RA patients were apparently higher than thoe in stable ones ( P ＜ 0. 01 ), meanwhile the sine case occurred between stable and control groups ( P ＜ 0. 01 ), the levels of plasma and synovial fluid MMP-3 showed positive relationship with CRP, DA S28 ( P ＜ 0.05 ) but none with RF( P ＞ 0.05 ), the plasma and synovial fluid levels of IL-17 also shown positive relationship with MMP-3, CRP, RF, DAS28 ( P ＜ 0.05 ). Conclusion IL-17 and MMP-3, which have abnormal elevations in synovial fluid and plasma from patients with RA, its levels were well correlated to the activity of RA and the elevated plasma IL-17 levels may be the useful markers for active RA.%目的:分析类风湿关节炎(RA)患者滑膜液及血浆中白细胞介素-17(IL-17)、基质金属蛋白酶-3(MMP-3)的水平及其临床意义.方法:应用酶联免疫吸附法(ELISA)检测RA患者滑膜液及血浆中IL-17及MMP-3的水平,分析IL-17及MMP-3与临床指标的关系.结果:RA患者滑膜液IL-17及MMP-3水平明显高于对照组(P均0.05);RA患者滑膜液及血浆中IL-17水平与MMP-3、CRP、RF及DAS28评分均呈正相关(P均<0.05).结论:RA患者的滑膜液及血浆中存在异常增高的IL-17及MMP-3,且其水平与病情活动相关,血浆IL-17可作为RA病情活动的参考指标之一.
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