唑来膦酸、聚乳酸-羟基乙酸聚合物、β-磷酸三钙复合支架修复去势大鼠股骨干骺端骨缺损的实验研究

摘要

目的 唑来膦酸、聚乳酸-羟基乙酸聚合物、β-磷酸三钙(ZA、PLGA、β-TCP)复合支架对去势大鼠股骨干骺端骨缺损的修复作用.方法 雌性SD大鼠经过双侧去卵巢手术后饲养3个月建立骨质疏松模型,随后在大鼠双侧股骨干骺端建立直径为3 mm圆形骨缺损,上述大鼠随机分为4组;分别置入ZA、PLGA支架,β-TCP支架和ZA、PLGA、β-TCP复合支架,不置入支架材料的为对照组.术后12周取材,通过Micro-CT扫描重建和病理组织学评价三组支架材料的成骨作用.结果 术后观察发现空白对照组骨缺损未修复,骨缺损断端硬化,而其他3组骨缺损均有不同程度修复.植入骨质疏松大鼠体内12周后,三组材料随着材料的降解均有新生骨长入,且三组新骨生成率均显著优于对照组(P＜0.05),缺损区域都有较高BV/TV、Tb.Th、Tb.N、Conn.D、骨矿化沉积率(MAR)和较低的Tb.Sp,其中以ZA、PLGA、β-TCP组最高.Micro-CT和病理组织学结果提示ZA、PLGA、β-TCP组骨修复效果较ZA、PLGA与β-TCP组更好.结论 ZA、PLGA、β-TCP复合支架具有明显促进去势大鼠股骨干骺端骨缺损修复的作用.%Objective To study the effect of zoledronic acid/poly (lactic-co-glycolic acid)/β-tricalcium phosphate (ZA/PLGA/ β-TCP) composite scaffold on the repair of femur metaphyseal defect in ovariectomized rats.Methods Ovariectomized female SD rats were fed for 3 months to establish osteoporosis model,then round bone defect of 3 mm diameter was established in bilateral femur metaphysis of rats.The rats were randomly divided into 4 groups.The ZA/PLGA scaffold was implanted through open operation into the bone defects in Group ZA/PLGA,Group β-TCP was implanted with β-TCP scaffold,Group ZA/PLGA/β-TCP was implanted with ZA/PLGA/β-TCP composite scaffold,and the control group did not have any implant.Twelve weeks after operation,osteogenesis was evaluated by Micro-CT scanning and histopathological evaluation.Results Observation found no repair but sclerosis of bone ends in the control group while bone repair of different extents was observed in the other 3 groups.Twelve weeks after the implantation,compared with the control group,the three implanted group had significantly greater new bone formation rate (P ＜ 0.05),and higher BV/TV,Tb.Th,Tb.N,Conn.D and bone mineralization deposition rate (MAR) and lower Tb.Sp at the defect areas,with the highest observed in the ZA/PLGA/β-TCP group.Micro-CT and histopathologicalresult showed that the ZA/PLGA/β-TCP group had better bone repair than the ZA/PLGA and the β-TCP groups.Conclusion ZA/ PLGA/β-TCP composite scaffolds can significantly improve the repair of femur metaphyseal bone defects in ovariectomized rats.