前列腺素E1对移植肝的保护作用

摘要

Objective　To study the protective effect of prostaglandin E1 (PGE1) on the liver in orthotopic liver transplantation rats. Methods　PGE1 was infused intravenously during orthotopic liver transplantation in recipient rats. Sham-operated and normal saline groups served as control groups. One-week survival rate and ultrastructure pathological changes in liver were observed. Results　The survival rate in the PGE1-treated group was obviously increased as compared with that in the control group. Electron microscopic examination revealed a dramatic elevation of hepatocellular lysosome number in the control groups, and bleb and rupture of lysosome membrane were also observed in the PGE1-trated group. Conclusions　PGE1 treatment during recipient operation could increase one-week survival rate, which might be related with its stabilizing effect on lysosome membrane.%目的探讨术中应用前列腺素E1（PGE1）对移植肝脏的保护作用。方法大鼠原位肝移植术中经颈内静脉灌注PGE1，设盐水和空白对照，观察术后1周存活率和肝脏超微结构变化。结果 PGE1治疗组术后1周存活率较对照组明显提高，电镜证实PGE1治疗组肝细胞内溶酶体膜无鼓泡和破溃现象。结论术中应用PGE1能显著提高大鼠肝移植后的1周存活率，其机制可能与PGE1稳定溶酶体膜的作用有关。