Objective: To investigate the effect of platelet-rich fibrin (PRF) in bone regeneration in experi-mental bone defect and to explore its reasons to provide a reference for its clinical research and appli-cations. Method: Six 8-12 month healthy male goats were used for this study and three round shaped defects(5mm diameter, 2mm deep) were formed in the buccal ridge of the bilateral mandibular anterior edentulous area by using the method of self control. The three defects were divided into three groups (Group A、Group B and Group C). Group A received PRF membranes as the experimental group; Group B received Haiao collagen membranes as the standard control group; Group C did not received any grafts as the blank control group. Animals were killed at 4、8 and 12 weeks after operation. The speci-mens were examined by gross observation、X-ray、HE histologic observation to compared the osteogenic effect. HPIAS-2000H high-resolution colour pathological graph-text analytical system was used to cal-culate the percentage of new formed bone area. SPSS 13.0 for statistical analysis. Results: At 4 weeks, there were less new bone formation, fewer osteoblasts, more fibers in the blank control group than those in the experimental group and standard control group. At 8 weeks, in the experimental group and stan-dard control group, the new formed bone thickened and connected into networks, the quantities of os-teoblasts were encreased and the fibrous tissue decreased. While in the blank control group there is rel-atively thin trabeculae, more fibrous tissue. At 12 weeks, the experimental group and standard control group showed visible lamellar structure and thicker trabeculae, while the blank control group showed no lamellar structure. Bone histomorphometry analysis: the percentage of new bone area at 4 weeks, 8 weeks and 12 weeks in the experimental group (35.92±3.89,60.72±4.53,74.73±3.64 respectively)were higher than those in the blanck control group (22.56 ±4.59,52.18 ±5.31,66.77 ±4.52 respectively), the difference was statistically significant (P<0.05). The percentage of new bone area at 4 weeks, 8 weeks and 12 weeks in the experimental group were not significantly different from those in the standard con-trol group(34.36±3.61,61.95±5.24,76.24±3.35 respectively)(P> 0.05). Conclusions: PRF is effective in promoting bone regeneration in the experimental bone defect.%目的：研究富血小板纤维蛋白在骨缺损修复中的作用，为临床研究和应用提供参考。方法：选用8-12月龄健康雄性山羊6只，采用自体对照的方法在羊的双侧下颌前部无牙区颊侧制备3个直径5mm，深2mm圆形骨缺损。将3个缺损分为ABC三组： A组放置富血小板纤维蛋白膜作为实验组， B组覆盖海奥修复膜作为标准对照组， C组不放置任何材料作为空白对照组。依次在4周、8周及12周处死动物，获取标本，进行大体标本观察、 X线影像观察、 HE切片观察，比较三组的成骨效果，并以HPIAS-2000H高清晰型多媒体彩色病理图文分析软件进行骨计量学分析，计算新生骨小梁面积百分比。 SPSS 13.0统计学软件进行统计分析。结果：组织切片显示，4周实验组和标准对照组缺损区新生骨小梁相对较多，表面有类骨质沉积，成骨细胞活跃，成排排列，纤维成分较少；空白对照组新生骨小梁稀少，成骨细胞数量较少，纤维成分较多。8周时实验组和标准对照组骨小梁增厚增多，相互连成网状，成骨细胞丰富，纤维成分较少；空白对照组骨小梁相对纤细，纤维成分较多。12周实验组和标准对照组可见明显板层结构，骨小梁粗大，空白对照组未见板层结构。骨形态计量学分析显示：实验组在4周、8周、12周时新生骨小梁面积百分比(35.92±3.89，60.72±4.53，74.73±3.64)均高于空白对照组(22.56±4.59，52.18±5.31，66.77±4.52)，差异有统计学意义(P<0.05)；与标准对照组(34.36±3.61，61.95±5.24，76.24±3.35)均无显著性差异(P>0.05)。结论：富血小板纤维蛋白在实验性骨缺损的修复中具有较好的促进成骨作用。
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