同种晶状体抗原免疫致兔白内障的实验研究

摘要

Objective：To establish cataract animal models induced by immunity of isologous lens antigens for future investigation.Methods：20 rabbits were divided into 2 groups. In group A, rabbits were immunized with isologous lens antigens(IL-Ags),+CFA while in group B rabbits were injected with PBS+CFA. The levels of special antibodies against IL-Ags, histopathology of eyes, lens opacity under slit-lamp microscope, and electroretinogram(ERG) were observed.Results：Of 8 animals in group A, levels of antibodies against IL-Ags rose with lens opacity, histo-pathological lesions and abnormal ERG.Conclusion：Animal models with cataract formation induced by immunity of isologous lens antigens provides clinical basis for further researchs. The successful rate of cataract animal model was 40% in this experiment.%目的:建立自身免疫性白内障动物模型，为今后白内障病因研究以及临床预防和治疗提供必需条件。 方法:采用同种晶状体抗原免疫家兔；观察血清特异性抗体水平，晶状体病理和裂隙灯下晶状体形态学变化以及视觉电生理改变。 结果:实验组动物血清特异抗体水平明显升高，经统计学处理有显著性意义(P＜0.05)；部分动物出现晶状体混浊和免疫原性病理损伤以及视觉电生理异常。结论：同种晶状体抗原免疫可造成眼部白内障样病变，即制成了可供进一步研究利用的自身免疫性白内障动物模型。