Rag-1基因敲除小鼠的眼部改变

摘要

Objective To evaluate the changes in the eyes of Rag-1 knockout mice. Methods Two groups of mice (10 in each group), a Rag-1 knockout group and a wild-type group, were used to investigate the differences in eyes by comparing and analyzing gene identification, refraction, elec-troretinogram (ERG), and ocular pathology. Results There were no differences between gene knockout mice and wild-type mice when the anterior of eyes, refraction and ocular pathology were analyzed. The ERG showed a significant difference in the a-wave and b-wave, which demonstrates the function of the retina. Conclusion Rag-1 knockout mice are ideal animal models for immune function research.%目的 观察Rag-1基因敲除鼠的眼部改变,为进一步研究Rag-1基因功能和其对眼球发育的影响打下一定的实验基础.方法 取Rag-1基网敲除型和野生型两种小鼠各10只,雌性,4～6周龄.通过基因鉴定、屈光检查、视网膜电图(ERG)检查、眼球病理分析,观察两种小鼠的眼部情况.结果 在眼前节、眼屈光和病理检查中,野生型和基因敲除型小鼠在结构上没有明显的差异,两者的屈光状态也无明显差异,均呈明显的远视状态.ERG主要反映视网膜的功能状态,两者的a波和b波没有明显的差异.结论 Rag-1基因敲除型小鼠是一种非常理想的研究眼免疫功能的动物模型.