神经损伤诱导蛋白-1对早期糖尿病大鼠视网膜髓样细胞黏附的调节作用

摘要

目的 观察神经损伤诱导蛋白(Ninj)-1对早期糖尿病大鼠视网膜髓样细胞黏附的调节作用.方法 实验研究.通过链脲佐菌素腹腔内注射诱导建立大鼠糖尿病模型.建模成功后2个月,取27只大鼠随机分为糖尿病+磷酸盐缓冲液(PBS)腹腔内注射组(B组)、糖尿病+抗Ninj-1腹腔内注射组(C组)、糖尿病+ IgG腹腔内注射组(D组),每组9只大鼠.取9只月龄匹配的正常大鼠作为对照组(A组).分别以丫啶橙视网膜白细胞造影和髓过氧化物酶(MPO)酶联免疫吸附试剂盒检测视网膜白细胞黏附数量及髓样细胞活性.4组间均数的比较采用单因素方差分析,均数间差异的多重比较采用LSD-t检验.结果 丫啶橙视网膜造影检测结果显示,A、B、C、D组大鼠视网膜白细胞数量分别为(49.66±13.51)、(153.66±20.43)、(85.33±15.03)、(156.33±11.53)个,差异有统计学意义(F=143.34,P=0.000),C组大鼠视网膜白细胞数量显著低于B组(P=0.000,95％可信区间为-82.68～-53.98).4组大鼠视网膜上清液中MPO的浓度分别为(15.66±2.08)、(27.66±2.51)、(18.02±2.01)、(26.66±3.21) μg/L,差异有统计学意义(F=17.61,P=0.010),C组大鼠视网膜组织中MPO水平显著低于B组(P=0.010,95％可信区间为-14.37～-4.95).结论 在体内实验条件下,Ninj-1可能对早期糖尿病大鼠视网膜髓样细胞黏附有一定的调节作用.%Objective To investigate the regulatory effects of ninjurin-1 on adhesion of myeloid cells in the retina at the early stage of diabetic rats.Methods Experimental study.The rat diabetic model was induced by intraperitoneal injection of streptozotocin.After 2 months of diabetes induction,27 diabetic rats were randomly chosen and assigned to 3 groups,including diabetes and phosphate buffered saline(PBS)injection group (group B),diabetes and anti-Ninj-1 injection group (group C) and diabetes and anti-IgG injection group (group D),with 9 rats in each group.Nine age matched health rats were chosen as control group(group A).Retinal leukostasis was quantified with acridine orange leukocyte fluorography.Retinal myeloid cell infiltration activity was measured by enzyme linked immunosorbent assay of myeloperoxidase (MPO).The differences of the mean values among the four groups were analyzed by one-factor analysis of variance.The multiple comparisons of the mean values among the four groups were analyzed by LSD-t analysis.Results According to the results of the acridine orange leukocyte fluorography,the numbers of leukocyte adhesion in the four groups were 49.66±13.51,153.66±20.43,85.33±15.03 and 156.33±11.53,respectively.The differences among them were significant(F=143.34,P =0.000).The numbers of leukocyte adhesion in the group C were significantly lower than that in group B (P =0.000,95％ CI:-82.68--53.98).The levels of retinal MPO in the four groups were (15.66 ± 2.08),(27.66 ± 2.51),(18.02 ± 2.01) and (26.66 ± 3.21) μg/L,respectively.The differences among them were significant(F =17.61,P =0.010).The level of retinal MPO in the group C was significantly lower than that in group B(P =0.010,95％ CI:-14.37--4.95).Conclusions Ninj-1 may play a role in the mediation of the adhesion of myeloid cell to the rat retina of early-stage of diabetes in vivo.