胃癌患者外周血Th17和Treg细胞的特异性转录因子与相关细胞因子的检测及临床意义

摘要

目的 了解胃癌患者外周血Th17和Treg细胞的转录因子及其相关细胞因子的表达水平,探讨其在胃癌发展进程中的作用.方法 收集57例胃癌患者、31例胃部良性疾病患者和40例健康志愿者的外周血,采用实时荧光定量逆转录聚合酶链反应(QRT-PCR)技术,检测外周血单个核细胞中Th17和Treg细胞的特异性转录因子RORγt和FoxP3 mRNA的表达水平;采用酶联免疫吸附试验(ELISA),检测胃癌患者血浆中IL-17、IL-23、转化生长因子β(TGF-β)和IL-10的浓度.结果 胃癌组FoxP3和RORγt mRNA的表达水平分别为2.349±0.491和0.794±0.304,明显高于健康对照组和良性疾病组(均P<0.05),FoxP3/RORγt比值也高于健康对照组和良性疾病组(P<0.05),而良性疾病组与健康对照组差异无统计学意义(P>0.05).中晚期胃癌组的FoxP3/RORγt比值明显高于早期胃癌组(P<0.05).胃癌组血浆中IL-17、IL-23、TGF-β和IL-10的水平明显高于健康对照组(P<0.05),中晚期胃癌组TGF-β和IL-10水平明显高于早期胃癌组(P<0.05).结论 胃癌患者存在高Th17和Treg细胞水平,且随着病程的进展Treg细胞仍维持强势,FoxP3/RORγt比值明显增高.监测胃癌患者外周血Th17和Treg细胞的特异性转录因子和相关细胞因子水平,有助于对病程的判断.%Objective To detect the expression levels of transcription factors and associated eytokines of Th17 and Treg cells in periphend blood mononuelear cells(PBMC)of patients with gastric cancer.and explore the possible pathological mechanism of these cells involved in the development of gastric cancer.Methods The mRNA levels of RORγt.FoxP3 in PBMC were determined by quantitative real-time PCR(QRT-PCR)from 57 patients with gastric cancer,31 patients with benign gastric illness and 40 healthy people.The concentration of IL-17,IL-23,TGF-β,IL-10 in plasma were detected by enzyme linked immtmosorbent assay(ELISA).Results Compared with healthy volunteers,patients with gastric cancer showed higher levels of RORγt and FoxP3 in PBMC(P<0.05).The ratio of FoxP3/RORγt in gastric cancer group was higher than that in the volunteer group and benign gastric illness group(P<0.05).The ratio of FoxP3/RORγt was higher in advanced disease than early disease(P<0.05).The expressions of IL-17,IL-23,TGF-β and IL-10 were higher in patients with gastric cancer than that in healthy volunteers(P<0.05).In addition,The expression of TGF-β and IL-10 were significantly increased in the advanced disease group than that in the early group(P<0.05),but IL-17 and IL-23 wag not significantly changed between the two groups(P>0.05).Conclusion There are higher levels of Th17 and Treg cells in gastric cancer patients.and it also shows a persistent predominant tendency of Treg cells and a reduced tendency of Th17cells in advanced disease.Detecting the expression of Th17/Treg transcription factor and related cytokines would contribute to the diagnosis and prediction of the disease development and prognosis.